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Proteinases in psoriatic arthritis

ABSTRACT: Characterize active synovial fluid (SF) serine proteinases in psoriatic arthritis (PsA) in comparison to osteoarthritis (OA) and rheumatoid arthritis (RA)

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Synovia

SUBMITTER: Omar Cruz

LAB HEAD: Vinod Chandran

PROVIDER: PXD022442 | Pride | 2021-03-29

REPOSITORIES: Pride

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			Action	DRS
	C1_1control.raw	Raw
	OA1_1.raw	Raw
	OA1_10.raw	Raw
	OA1_11.raw	Raw
	OA1_12.raw	Raw

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Publications

Proteinase-Mediated Macrophage Signaling in Psoriatic Arthritis.

Abji Fatima F Rasti Mozhgan M Gómez-Aristizábal Alejandro A Muytjens Carla C Saifeddine Mahmoud M Mihara Koichiro K Motahhari Majid M Gandhi Rajiv R Viswanathan Sowmya S Hollenberg Morley D MD Oikonomopoulou Katerina K Chandran Vinod V

Frontiers in immunology 20210308

<h4>Objective</h4>Multiple proteinases are present in the synovial fluid (SF) of an arthritic joint. We aimed to identify inflammatory cell populations present in psoriatic arthritis (PsA) SF compared to osteoarthritis (OA) and rheumatoid arthritis (RA), identify their proteinase-activated receptor 2 (PAR2) signaling function and characterize potentially active SF serine proteinases that may be PAR2 activators.<h4>Methods</h4>Flow cytometry was used to characterize SF cells from PsA, RA, OA pati ...[more]

PMID: 33763056

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Proteinase-mediated macrophage signalling in psoriatic arthritis

Project description:To identify the cell populations in synovial fluid (SF) from psoriatic arthritis (PsA) patients, single cell 3’-RNA-sequencing of cells from PsA SF samples (n=3) was performed using the 10X Genomics platform. Twelve main clusters of cells were predicted and the most abundant clusters had a predominance of monocytes/macrophages gene expression patterns.

2020-12-15 | GSE161500 | GEO

Changes in neutrophil gene expression in psoriatic arthritis patients in response to sekukinumab

Project description:We report changes in gene expression in human peripheral blood neutrophils from patients with psoriatic arthritis (PsA) before and 12-weeks after treatment with Secukinumab (SKB, 150mg). All patients achieved a good PSARC (Psoriatic Arthritis Response Criteria) and PASI 90 (Psoriasis Area and Severity Index) response to treatment. We also report gene expression in healthy control neutrophils compared to patients wth psoriatic arthritis.

2023-01-06 | GSE179800 | GEO

10x single cell gene expression and V(D)J immune profiling of T cells from the blood, synovial fluid and synovial tissue in psoriatic arthritis patients

Project description:This study shows the pronounced expansion of CD8 T cell clones at sites of active inflammation within the joints of psoriatic arthritis (PsA) patients compared to blood, and characterises the gene expression of expanded clones using droplet based single cell RNA sequencing. Mononuclear cells were separated from freshly acquired paired peripheral blood and synovial fluid samples from 3 PsA patients using density gradient separation, then enriched for CD4 and CD8 T cells using FACS. Additionally, CD45+ leukocytes were enriched by FACS from the cryopreserved synovial tissue of 2 further PsA patients. All cells were then processed using a 10x Chromium Controller and sequenced by Illumina HiSeq 4000 (peripheral blood / synovial fluid) or NovaSeq with S2 flowcell (synovial tissue).

2020-09-07 | E-MTAB-9492 | biostudies-arrayexpress

Human Arthritic Synovial Fluid LC-MS/MS

Project description:In the present study, synovial fluid (SF) samples from inflammatory arthritis (IA) patients were subjected to a label-free, mass spectrometry-based approach for the identification and quantification of IA-specific proteins and peptides. The discovery-phase project generated a warehouse of proteomic and peptidomic data providing a basis for the identification of candidates with the greatest potential to serve as novel biomarkers specific to inflammatory arthritides. A total of 11 protein candidates and 15 peptide candidates, originating from 4 proteins, were selected for follow-up studies.

2019-11-12 | PXD011872 | Pride

Single cell analysis reveals multiple subsets of CD8+ TRM and non-TRM cells with a type 17 signature in the inflamed psoriatic arthritis joint

Project description:CD69+CD103+ tissue-resident memory T-cells (TRM) are increasingly recognised as important drivers of inflammation. To decipher their potential role in inflammatory arthritis, we applied single cell, high-dimensional profiling (CyTOF and scRNAseq) to T-cells isolated from the joint of patients with psoriatic arthritis (PsA) or rheumatoid arthritis (RA). We identified three broad groups of synovial CD8+ TRM cells: cytotoxic and Treg-like TRM cells were present in the inflamed joints of patients with both PsA and RA, while CD161+CXCR6+ type 17-like TRM cells with a pro-inflammatory cytokine profile (IL-17A+TNFa+IFNg+) and a distinct transcriptomic signature and a polyclonal, but distinct TCR repertoire, were specifically enriched in the inflamed joints of patients with PsA. Type 17-like cells were also enriched in non-TRM CD8+ T-cells in PsA compared to RA. These findings add substantively to the accumulating evidence that the immunopathology of PsA and RA is different, with a particular role for type 17 cells in PsA.

2023-05-17 | GSE216914 | GEO

Altered fecal metabolomics and potential biomarkers of psoriatic arthritis differing from rheumatoid arthritis

Project description:Psoriatic arthritis (PsA) is a chronic inflammatory joint disease, and the diagnosis is quite difficult due to the unavailability of reliable clinical markers. This study aimed to investigate the fecal metabolites in PsA by comparison with rheumatoid arthritis (RA), and to identify potential diagnostic biomarkers for PsA. The metabolic profiles of the fecal samples from 27 PsA and 29 RA patients as well as 36 healthy controls (HCs) were performed on ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). And differentially altered metabolites were screened and assessed using multivariate analysis for exploring the potential biomarkers of PsA. The results showed that 154 fecal metabolites were significantly altered in PsA patients when compared with HCs, and 45 metabolites were different when compared with RA patients. A total of 14 common differential metabolites could be defined as candidate biomarkers. Furthermore, a support vector machines (SVM) model was performed to distinguish PsA from RA patients and HCs, and 5 fecal metabolites, including α/β-turmerone, glycerol 1-hexadecanoate, dihydrosphingosine, pantothenic acid and glutamine, were determined as biomarkers for PsA. Through the metabolic pathways analysis, we found that the abnormality of amino acid metabolism, bile acid metabolism and lipid metabolism might contribute to the occurrence and development of PsA. In summary, our research provided ideas for the early diagnosis and treatment of PsA by identifying fecal biomarkers and analyzing metabolic pathways.

2022-02-23 | MTBLS4206 | MetaboLights

Transcriptional analysis of normal and pathological synovial fibroblasts

Project description:The aim of this study was to compare gene expression between two pathological groups of human synovial fibroblasts (SF) from rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissues with normal SF from healthy individuals (HSF). We used microarray expression profiling in SF cultured from OA, RA and normal synovial tissues. We found larger numbers of transcripts with differential expression in OASF compared to the other groups than in RASF compared to HSF. This data demonstrate that cultured OASF display a more robust transcriptomic profile than RASF when compared to HSF. Synovial fibroblasts were obtained from 9 patients with rheumatoid arthritis (RASF), 11 sex and age matched adult healthy donors (HSF) and 11 sex and age matched patients with OA (OASF). SF were collected under similar subconfluent conditions 24h after serum addition. 31 microarray data were used for determine the statistical significance (p value) of the differences in gene expression.

2011-10-24 | E-GEOD-29746 | biostudies-arrayexpress

Genetic Analysis of Psoriasis and Psoriatic Arthritis

Project description:<p>This study is a genome-wide study of genetic associations with psoriatic arthritis (PsA), an inflammatory musculoskeletal condition that develops in up to 30% of people who have chronic psoriasis skin lesions. 1,526 cases affected with psoriatic arthritis and 1,508 unaffected controls were recruited and typed on the Illumina HumanOmni1-Quad BeadChip array. After application of quality control measures to both samples and SNPs, genotypes for 791,217 autosomal SNPs were available for 1,430 PsA cases and 1,417 controls. Imputation using phased haplotypes for the EUR subpopulation of release 3, phase 1 of the 1000 Genomes Project as a reference, resulted in genotypes for 11,532,644 SNPs and 918,976 indels passing our imputation quality threshold (Mach r<sup>2</sup> ≥ 0.3) that were available for downstream analysis. Association at a genome-wide threshold of significance was found for five regions (near genes <i>TNIP1</i>, <i>IL12B</i>, <i>HLA-C</i>, <i>TRAF3IP2</i>, and <i>TYK2</i>).</p>

| phs000982 | dbGaP