Proteomics

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Discovery of biomarkers in radioresistant MCF7R breast cancer xenografts

ABSTRACT: Radioresistance of tumors is not completely understood. Our previous studies have shown that MCF-7 breast cancer cell line exposed to 6 Gy and allowed to recover for 7 days (D7-6G) developed radio-resistance. In this study, we have tested the ability of these cells to form tumors in SCID mice and characterized these tumors by proteomic analyses. Untreated (MCF-C) and D7-6G cells (MCF-R) were injected s.c. in SCID mice and tumor growth monitored. On day 18, the mice were sacrificed and proteins were extracted from tumor tissues label free proteomic analyses was carried out by LC-MS.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Bhavani Shankar  

LAB HEAD: Dr. Bhavani Shankar

PROVIDER: PXD022506 | Pride | 2021-01-04

REPOSITORIES: Pride

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Publications

Proteomic analysis of radio-resistant breast cancer xenografts: Increased TGF-β signaling and metabolism.

Yadav Poonam P   Pandey Vipul K VK   Shankar Bhavani S BS  

Cell biology international 20201231 4

Our previous studies have shown that MCF-7 breast cancer cell line exposed to 6 Gy and allowed to recover for 7 days (D7-6G) developed radio-resistance. In this study, we have tested the ability of these cells to form tumors in severe combined immunodeficiency (SCID) mice and characterized these tumors by proteomic analyses. Untreated (MCF-C) and D7-6G cells (MCF-R) were injected s.c. in SCID mice and tumor growth monitored. On Day 18, the mice were killed and tumor tissues were fixed in formali  ...[more]

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