Dataset Information

Quantitative proteomics characterization of proteins modified by very long chain fatty acids (VLCFAs) under necroptosis

ABSTRACT: Necroptosis is a caspase-independent form of regulated cell death that is characterized by membrane permeabilization and rupture. This membrane rupture is responsible for the inflammatory properties of necroptosis and is critical for disease states involving this process. In efforts to understand how lipids might contribute to necroptosis, we previously showed that saturated very long chain fatty acids (VLCFAs) are functionally involved in this process, potentially through protein fatty acylation. Here we define the scope of protein acylation by saturated VLCFAs during necroptosis using a clickable lipid analog and IonStar quantitative proteomics.As a result, a total of 1672 proteins were quantified with high precision and no missing data across samples in the same condition. 1267 were detected with higher abundances in control cells and 405 were detected with higher abundance in necroptotic conditions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Shichen Shen

LAB HEAD: Jun Qu

PROVIDER: PXD022527 | Pride | 2021-05-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	191107_Ekin_alkFA_C1_180min_120k_clm156_65cm_HCD_IT_val50_HCD30_4ul_01.msf	Msf
	191107_Ekin_alkFA_C1_180min_120k_clm156_65cm_HCD_IT_val50_HCD30_4ul_01.raw	Raw
	191107_Ekin_alkFA_C1_180min_120k_clm156_65cm_HCD_OT_val50_HCD30_4ul_01.msf	Msf
	191107_Ekin_alkFA_C1_180min_120k_clm156_65cm_HCD_OT_val50_HCD30_4ul_01.raw	Raw
	191107_Ekin_alkFA_C2_180min_120k_clm156_65cm_HCD_IT_val50_HCD30_4ul_01.msf	Msf

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Publications

Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.

Pradhan Apoorva J AJ Lu Daniel D Parisi Laura R LR Shen Shichen S Berhane Ilyas A IA Galster Samuel L SL Bynum Kiana K Monje-Galvan Viviana V Gokcumen Omer O Chemler Sherry R SR Qu Jun J Kay Jason G JG Atilla-Gokcumen G Ekin GE

Cell chemical biology 20210412 9

Necroptosis is a form of cell death characterized by receptor-interacting protein kinase activity and plasma membrane permeabilization via mixed-lineage kinase-like protein (MLKL). This permeabilization is responsible for the inflammatory properties of necroptosis. We previously showed that very long chain fatty acids (VLCFAs) are functionally involved in necroptosis, potentially through protein fatty acylation. Here, we define the scope of protein acylation by saturated VLCFAs during necroptosi ...[more]

PMID: 33848465

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