Proteomics

Dataset Information

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Proteomic analysis of FPA interactors


ABSTRACT: Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat receptor genes (NLRs) are found in plant genomes and provide disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, controlling their functional expression and impacting immunity. Using long-read nanopore direct RNA sequencing we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in immunity.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Matthew Parker  

LAB HEAD: Gordon Simpson

PROVIDER: PXD022684 | Pride | 2020-12-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Araport11_genes.201606.pep.repr.fasta Fasta
FPA_1_1.raw Raw
FPA_1_2.raw Raw
FPA_1_3.raw Raw
FPA_1_4.raw Raw
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