Proteomics

Dataset Information

0

Comparative analysis of PAD4 and SAG101 interactors upon activation of NLR immunity in Arabidopsis thaliana


ABSTRACT: Intracellular NLR receptors with a central nucleotide-binding domain allow plants to detect specific pathogen-secreted proteins and initiate immune signaling. A family of conserved Enhanced disease susceptibility 1 proteins with a lipase-like and the EP domain transduces the signal from activated NLRs downstream. This family includes EDS1, PAD4 and SAG101 that form mutually exclusive heterodimers EDS1-PAD4 and EDS1-SAG101. Despite sequence similarities, PAD4 and SAG101 control different aspects of NLR immune signaling. More specifically, PAD4 and SAG101 regulate resistance and cell death outputs of the NLR receptor pair RRS1-RPS4, respectively. Also, PAD4 and SAG101 genetically cooperate with distinct downstream signaling NLRs - ADR1 and NRG1. The main aim of this IP-LC/MS project is to assess whether PAD4 and SAG101 form complexes with ADR1 and NRG1 and to gain insight into mechanisms of the genetically established functional links between EDS1 family proteins and downstream signaling NLRs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Sara Christina Stolze  

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD021301 | Pride | 2021-04-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
200605_JP_DiLa_B_01.raw Raw
200605_JP_DiLa_B_02.raw Raw
200605_JP_DiLa_B_03.raw Raw
200605_JP_DiLa_B_04.raw Raw
200605_JP_DiLa_B_05.raw Raw
Items per page:
1 - 5 of 14
altmetric image

Publications


Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 "helper" NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) and phytoalexin-deficient 4 (PAD4) lipase-like proteins to mediate signalling from TIR domain NLR receptors (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present g  ...[more]

Similar Datasets

2022-03-17 | PXD029477 | Pride
2021-07-05 | PXD026120 | Pride
2021-04-06 | PXD025202 | Pride
2016-09-27 | GSE80585 | GEO
2020-12-16 | PXD022684 | Pride
2024-07-20 | PXD054061 | Pride
2006-02-15 | E-MEXP-546 | biostudies-arrayexpress
2013-02-07 | E-GEOD-24766 | biostudies-arrayexpress
2019-08-04 | GSE118352 | GEO
2019-08-04 | GSE118356 | GEO