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Proteomic and phosphoproteomic profiling of human myoepithelial and luminal epithelial cells

ABSTRACT: The aim of the project is to perform deep proteomic and phosphoproteomic profiling of luminal epithelial and myoepithelial cells from the mammary gland of young vs. older women.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Antigoni Manousopoulou

LAB HEAD: Spiros D. Garbis

PROVIDER: PXD022691 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	07Jan20_LEPs__uniqueonly_2up95_1up99_50coisolation_proteins_GeneSymbol.csv	Csv
	07feb20_LEPs_phospho_proteomics_MS2datasets.csv	Csv
	07feb20_MEPs_phospho_proteomics_MS2datasets.csv	Csv
	10jan20_MEPs_95_50_unique.csv	Csv
	Antigoni_21dec19_LEP_Fenta.raw	Raw

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Publications

Deep proteome profiling of human mammary epithelia at lineage and age resolution.

Hinz Stefan S Manousopoulou Antigoni A Miyano Masaru M Sayaman Rosalyn W RW Aguilera Kristina Y KY Todhunter Michael E ME Lopez Jennifer C JC Sohn Lydia L LL Wang Leo D LD LaBarge Mark A MA

iScience 20210823 9

Age is the major risk factor in most carcinomas, yet little is known about how proteomes change with age in any human epithelium. We present comprehensive proteomes comprised of >9,000 total proteins and >15,000 phosphopeptides from normal primary human mammary epithelia at lineage resolution from ten women ranging in age from 19 to 68 years. Data were quality controlled and results were biologically validated with cell-based assays. Age-dependent protein signatures were identified using differe ...[more]

PMID: 34522866

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