Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic and phosphoproteomic profiling of human myoepithelial and luminal epithelial cells

ABSTRACT: The aim of the project is to perform deep proteomic and phosphoproteomic profiling of luminal epithelial and myoepithelial cells from the mammary gland of young vs. older women.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Antigoni Manousopoulou

LAB HEAD: Spiros D. Garbis

PROVIDER: PXD022691 | Pride | 2022-02-16

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	07Jan20_LEPs__uniqueonly_2up95_1up99_50coisolation_proteins_GeneSymbol.csv	Csv
	07feb20_LEPs_phospho_proteomics_MS2datasets.csv	Csv
	07feb20_MEPs_phospho_proteomics_MS2datasets.csv	Csv
	10jan20_MEPs_95_50_unique.csv	Csv
	Antigoni_21dec19_LEP_Fenta.raw	Raw

Items per page:

1 - 5 of 42

Publications

Deep proteome profiling of human mammary epithelia at lineage and age resolution.

Hinz Stefan S Manousopoulou Antigoni A Miyano Masaru M Sayaman Rosalyn W RW Aguilera Kristina Y KY Todhunter Michael E ME Lopez Jennifer C JC Sohn Lydia L LL Wang Leo D LD LaBarge Mark A MA

iScience 20210823 9

Age is the major risk factor in most carcinomas, yet little is known about how proteomes change with age in any human epithelium. We present comprehensive proteomes comprised of >9,000 total proteins and >15,000 phosphopeptides from normal primary human mammary epithelia at lineage resolution from ten women ranging in age from 19 to 68 years. Data were quality controlled and results were biologically validated with cell-based assays. Age-dependent protein signatures were identified using differe ...[more]

PMID: 34522866

Similar Datasets

Proteomic and phosphoproteomic profiling of aging human mammary myoepithelial and luminal epithelial cells

Project description:The aim of this project is to examine age-dependent changes in the proteomes and phosphoproteomes of normal human mammary luminal epithelial and myoepithelial cells.

2021-11-03 | PXD027540 | Pride

GATA-3 maintains luminal cell differentiation in the mammary gland and in breast cancer

2006-10-24 | GSE5602 | GEO

GATA-3 maintains luminal cell differentiation in the mammary gland and in breast cancer

Project description:We have identified GATA-3 as a critical regulator of luminal cell differentiation in the mammary gland. Acute loss of GATA-3 in the adult mammary gland leads to an expansion of an undifferentiated luminal epithelium and the formation of a multi-layered epithelium. Here we report microarray analysis of mammary glands that have undergone acute loss of GATA-3 Adult GATA-3flox/flox; WAP-rtTA-Cre and GATA-flox/+; WAP-rtTA-Cre mice were administered doxyxcline for 5 days and their mammary glands harvested. Total RNA was extracted by the Trizol method. Het mammary gland total RNA was labeled with Cy5 while Null mammary gland total RNA was labeled with Cy3. Microarray hybridization was performed on spotted oligonucleotide microarrays with 38,000 features. Lowess print-tip normalization and analysis was performed on the Acuity software package (V 4.0)

2010-07-01 | E-GEOD-5602 | biostudies-arrayexpress

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [04_cut_run_TRPS1]

Project description:The GATA transcription factor TRPS1 is a context-dependent oncogene in breast cancer. In the mammary gland, TRPS1 activity is restricted to the luminal population and sustains the initial branching of the mammary ducts at puberty as well as the lactogenic differentiation during pregnancy. Its function in the resting mammary gland however, remains unclear. To evaluate whether it could be a target for cancer therapy, we investigated TRPS1 function in the healthy resting mammary gland using a conditional ubiquitous depletion mouse model where long-term depletion does not affect fitness. we show that TRPS1 activity is critical to maintain a functional luminal progenitor compartment and that this involves the repression of the SRF/MRTF and the YAP/TAZ transcriptional program. Mechanistically, this repression involves Krt8-dependent RhoA modulation. Our work uncovers a hitherto undisclosed function of TRPS1 in luminal progenitors which is intrinsically linked to mechanotransduction in the mammary gland. It also provides new insights into the oncogenic functions of TRPS1 since luminal progenitors are likely the cells of origin of many breast cancers.

2024-05-24 | GSE243510 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [03_scatac_shTRPS1_organoids]

2024-05-24 | GSE243509 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [02_citeseq_shTRPS1_mmg]

2024-05-24 | GSE243508 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01d_bulk_rna_seq_lum_prog_vs_mature]

2024-05-24 | GSE243507 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01b_bulk_rna_seq_luminal_vs_basal]

2024-05-24 | GSE243505 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01a_bulk_rna_seq_PCX_Cre_shTRPS1]

2024-05-24 | GSE243504 | GEO

TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [01c_bulk_rna_seq_K8-CreER_shTRPS1]