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Proteomics and phosphoproteomics of SARS-CoV-2 and IAV treated APCs

ABSTRACT: The clinical course of SARS-CoV-2 infection is highly variable with a subset of patients developing severe COVID-19 and acute respiratory distress syndrome (ARDS). COVID-19 induced lung injury and respiratory failure appears to be driven by dysregulated immune responses, yet the exact mechanisms remain unknown. Here, we analyzed monocytes isolated from healthy donors treated with SARS-CoV-2, influenza A (Panama strain) or TLR7/8 agonist R848. Notably, overnight exposure to SARS-CoV-2, but not influenza A virus, induced a profibrotic signature, characterized by high expression of known fibrogenic factors like TGFB1, SPP1 and LGMN, and showed highly significant similarity with profibrotic macrophage populations identified in idiopathic pulmonary fibrosis (IPF). In conclusion, SARS-CoV-2 triggers profibrotic macrophage responses, and ARDS-associated lung fibrosis.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2 Influenza A Virus (a/panama/2007/1999(h3n2))

TISSUE(S): Peripheral Blood Mononuclear Cell

DISEASE(S): Covid-19,Influenza

SUBMITTER: Tommaso Mari

LAB HEAD: Matthias Selbach

PROVIDER: PXD022709 | Pride | 2021-11-29

REPOSITORIES: Pride

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			Action	DRS
	Gonzo_20200414_TM_HStmtpro_CoVPanMono_fr1.raw	Raw
	Gonzo_20200414_TM_HStmtpro_CoVPanMono_fr10.raw	Raw
	Gonzo_20200414_TM_HStmtpro_CoVPanMono_fr11.raw	Raw
	Gonzo_20200414_TM_HStmtpro_CoVPanMono_fr12.raw	Raw
	Gonzo_20200414_TM_HStmtpro_CoVPanMono_fr13.raw	Raw

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