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Optimization of Proteomics Sample Preparation for Forensic Analysis of Skin Samples

ABSTRACT: We present an efficient protein extraction and in-solution enzymatic digestion protocol optimized for mass spectrometry-based proteomics studies of human skin samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Maryam Baniasad

LAB HEAD: Michael Alan Freitas

PROVIDER: PXD022720 | Pride | 2021-11-02

REPOSITORIES: Pride

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			Action	DRS
	MB_IARPA_Method_PM_37C_16HR_1.mgf	Mgf
	MB_IARPA_Method_PM_37C_16HR_1.mzML	Mzml
	MB_IARPA_Method_PM_37C_16HR_3.mgf	Mgf
	MB_IARPA_Method_PM_37C_16HR_3.mzML	Mzml
	MB_IARPA_Method_PM_37C_16hr_1.msf	Msf

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Publications

Optimization of proteomics sample preparation for forensic analysis of skin samples.

Baniasad Maryam M Reed Andrew J AJ Lai Stella M SM Zhang Liwen L Schulte Kathleen Q KQ Smith Alan R AR LeSassier Danielle S DS Weber Katharina L KL Hewitt F Curtis FC Woerner August E AE Gardner Myles W MW Wysocki Vicki H VH Freitas Michael A MA

Journal of proteomics 20210901

We present an efficient protein extraction and in-solution enzymatic digestion protocol optimized for mass spectrometry-based proteomics studies of human skin samples. Human skin cells are a proteinaceous matrix that can enable forensic identification of individuals. We performed a systematic optimization of proteomic sample preparation for a protein-based human forensic identification application. Digestion parameters, including incubation duration, temperature, and the type and concentration o ...[more]

PMID: 34481086

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