Proteomics

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RawBeans: a simple, vendor independent, raw-data quality control tool


ABSTRACT: Every laboratory performing mass spectrometry based proteomics strives to generate high quality data. Among the many factors that influence the outcome of any experiment in proteomics is performance of the LC-MS system, which should be monitored continuously. This process is termed quality control (QC). We present an easy to use, rapid tool, which produces a visual, HTML based report that includes the key parameters needed to monitor LC-MS system perfromance. The tool, named RawBeans, can generate a report for individual files, or for a set of samples from a whole experiment. We anticipate it will help proteomics users and experts evaluate raw data quality, independent of data processing. The tool is available here: https://bitbucket.org/incpm/prot-qc/downloads.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yishai Levin  

LAB HEAD: Yishai Levin

PROVIDER: PXD022816 | Pride | 2021-03-11

REPOSITORIES: Pride

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RawBeans: A Simple, Vendor-Independent, Raw-Data Quality-Control Tool.

Morgenstern David D   Barzilay Rotem R   Levin Yishai Y  

Journal of proteome research 20210303 4


Every laboratory performing mass-spectrometry-based proteomics strives to generate high-quality data. Among the many factors that impact the outcome of any experiment in proteomics is the LC-MS system performance, which should be monitored within each specific experiment and also long term. This process is termed quality control (QC). We present an easy-to-use tool that rapidly produces a visual, HTML-based report that includes the key parameters needed to monitor the LC-MS system performance, w  ...[more]

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