Dataset Information

Application of harmonic FT-ICR-MS signal acquisition for improved proteome research

ABSTRACT: Proteomics experiments benefit from having both high resolving power and high scan speed. Harmonic detection provides a method to obtain twice the resolution for the same data acquisition period, or the same resolution in half the data acquisition period. We show that in a complex hela cell lysate sample, that we obtain 36% to 45% more unique peptides detected using harmonic detection with enhanced scan rate or resolution respectively.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sung-Gun Park

LAB HEAD: James E. Bruce

PROVIDER: PXD022838 | Pride | 2022-08-12

REPOSITORIES: Pride

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Application of frequency multiple FT-ICR-MS signal acquisition for improved proteome research.

Park Sung-Gun SG Mohr Jared P JP Anderson Gordon A GA Bruce James E JE

International journal of mass spectrometry 20210319

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) coupled with liquid chromatography (LC) is a powerful combination useful in many research areas due to the utility of high mass resolving power and mass measurement accuracy for studying highly complex samples. Ideally, every analyte in a complex sample can be subjected to accurate mass MS/MS analysis to aid in identification. FT-ICR MS can provide high mass resolving power and mass accuracy at the cost of long data acquisit ...[more]

PMID: 33897275

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