Dataset Information

Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients

ABSTRACT: The relationship between the microbial changes with clinical-pathological outcomes are still far from being conclusive. Herein, we investigate the ability of metagenomics (MG) and metaproteomics (MP) saliva data in distinguishing C, L0 and L1 patients. For that, we combined two strategies using MG analysis using 16S rDNA sequencing of saliva cells, and MP analysis using liquid chromatography tandem mass spectrometry of saliva supernatant and cells.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Adriana Franco Paes Leme

LAB HEAD: Adriana Franco Paes Leme

PROVIDER: PXD022859 | Pride | 2022-11-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 52

Publications

Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients.

Granato Daniela C DC Neves Leandro X LX Trino Luciana D LD Carnielli Carolina M CM Lopes Ariane F B AFB Yokoo Sami S Pauletti Bianca A BA Domingues Romênia R RR Sá Jamile O JO Persinoti Gabriella G Paixão Douglas A A DAA Rivera César C de Sá Patroni Fabio M FM Tommazetto Geizecler G Santos-Silva Alan R AR Lopes Márcio A MA de Castro Gilberto G Brandão Thaís B TB Prado-Ribeiro Ana Carolina AC Squina Fabio M FM Telles Guilherme P GP Paes Leme Adriana F AF

Biochimica et biophysica acta. Proteins and proteomics 20210409 8

Saliva is a biofluid that maintains the health of oral tissues and the homeostasis of oral microbiota. Studies have demonstrated that Oral squamous cell carcinoma (OSCC) patients have different salivary microbiota than healthy individuals. However, the relationship between these microbial differences and clinicopathological outcomes is still far from conclusive. Herein, we investigate the capability of using metagenomic and metaproteomic saliva profiles to distinguish between Control (C), OSCC w ...[more]

PMID: 33839314

Similar Datasets

Project description:BackgroundEpithelial-mesenchymal transition (EMT) plays a crucial role in the progression and aggressiveness of colorectal carcinoma. E-cadherin is the best-characterized molecular marker of EMT, but its prognostic significance for patients with CRC remains inconclusive.MethodologyEligible studies were searched from the PubMed, Embase and Web of Science databases. Correlation between E-cadherin expression and clinicopathological features and prognosis was analyzed. Subgroup analysis was also performed according to study location, number of patients, quality score of studies and cut-off value.Principal findingsA total of 27 studies comprising 4244 cases met the inclusion criteria. Meta-analysis suggested that downregulated E-cadherin expression had an unfavorable impact on overall survival (OS) of CRC (n?=?2730 in 14 studies; HR?=?2.27, 95%CI: 1.63-3.17; Z?=?4.83; P?=?0.000). Subgroup analysis indicated that low E-cadherin expression was significantly associated with worse OS in Asian patients (n?=?1054 in 9 studies; HR?=?2.86, 95%CI: 2.13-3.7, Z?=?7.11; P?=?0.000) but not in European patients (n?=?1552 in 4 studies; HR?=?1.14, 95%CI: 0.95-1.35, Z?=?1.39; P?=?0.165). In addition, reduced E-cadherin expression indicated an unfavorable OS only when the cut off value of low E-cadherin expression was >50% (n?=?512 in 4 studies; HR?=?2.08, 95%CI 1.45-2.94, Z?=?4.05; P?=?0.000). Downregulated E-cadherin expression was greatly related with differentiation grade, Dukes' stages, lymphnode status and metastasis. The pooled OR was 0.36(95%CI: 0.19-0.7, Z?=?3.03, P?=?0.002), 0.34(95%CI: 0.21-0.55, Z?=?6.61, P?=?0.000), 0.49(95%CI: 0.32-0.74, Z?=?3.02, P?=?0.002) and 0.45(95%CI: 0.22-0.91, Z?=?3.43, P?=?0.001), respectively.ConclusionsThis study showed that low or absent E-cadherin expression detected by immunohistochemistry served as a valuable prognostic factor of CRC. However, downregulated E-cadherin expression seemed to be associated with worse prognosis in Asian CRC patients but not in European CRC patients. Additionally, this meta-analysis suggested that the negative threshold of E-cadherin should be >50% when we detected its expression in the immunohistochemistry stain.

			Action	DRS
	09mar_DG_C11.raw	Raw
	09mar_DG_C13.raw	Raw
	09mar_DG_S100.raw	Raw
	09mar_DG_S102.raw	Raw
	09mar_DG_S108.raw	Raw