Proteomics

Dataset Information

0

Stradard protein and yeast top-down data


ABSTRACT: We developed a novel dissociation strategy in the data acquisition process, the Mesh strategy to dissociate multiple charge states of one proteoform with stepped HCD (high collision energy dissociation). We proved that the ‘Mesh’ strategy could generate fragments with improved sequence coverage and it could also improve identification rate in top-down proteomics analysis for complicated samples. The strategy is implemented within an an open source real-time instrument control software program called MetaDrive to perform real time deconvolution and precursor selection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Lei Lu  

LAB HEAD: Lei Lu

PROVIDER: PXD022941 | Pride | 2022-06-09

REPOSITORIES: Pride

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Publications

Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics.

Lu Lei L   Scalf Mark M   Shortreed Michael R MR   Smith Lloyd M LM  

Journal of the American Society for Mass Spectrometry 20210323 6


Top-down proteomics is a key mass spectrometry-based technology for comprehensive analysis of proteoforms. Proteoforms exhibit multiple high charge states and isotopic forms in full MS scans. The dissociation behavior of proteoforms in different charge states and subjected to different collision energies is highly variable. The current widely employed data-dependent acquisition (DDA) method selects a narrow <i>m</i>/<i>z</i> range (corresponding to a single proteoform charge state) for dissociat  ...[more]

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