Proteomics

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Pseudomonas syringae effector HopZ3 suppresses PTO immune pathway in tomato by acetylation


ABSTRACT: The plant pathogen Pseudomonas syringae secretes multiple effectors to suppress plant defenses. Some effectors are recognized by plants while others can function to suppress the resulting immune responses. HopZ3 of Psy B728a is an aminotransferase targeting multiple components of both plant immune complexes and Psy effectors, leading to the activation of these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and Psy effectors AvrRpm1 and AvrB3 s. In P. syringae resistant tomato, the main immune complex includes PRF and PTO. We found that HopZ3 acts as a virulence factor on tomato by suppressing defenses triggered by Psy AvrPto1. HopZ3 interacts with members of the PTO complex and acetylates PTO, AvrPto1, SlRIPK and SlRIN4s in vitro and in planta. Mapping HopZ3-dependent acetylation sites in planta revealed that AvrPto1 is modified on histidine, serine and threonine residues. The most highly acetylated AvrPto1 residues are T91 and S94 in a loop essential for interaction with PTO. HopZ3 also acetylates, in planta, T204 and T199 in the PTO activation loop which is important for kinase activity and recognition of AvrPto1. In agreement with these findings, we showed that acetylation of either AvrPto1 or PTO by HopZ3 reduces their binding in vitro. Mutation of acetylation sites T91A or H125A/H135A in AvrPto1 does not affect PTO binding in vitro but decreases AvrPto1 recognition in tomato in the absence of HopZ3 and increases bacterial growth. We also found decreased in planta phosphorylation in the presence of HopZ3 for some residues in AvrPto1 and PTO involved in immune signaling. Moreover, acetylation of SlRIPK by HopZ3 reduces its activity as well as phosphorylation of HopZ3 and SlRIN4s. Psy modulation of both its own avirulence effectors and their plant targets leads to decreased defenses and increased susceptibility to infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Pseudomonas Syringae Pv. Syringae

SUBMITTER: Donald Wolfgeher  

LAB HEAD: Dr. Jean T. Greenberg

PROVIDER: PXD022953 | Pride | 2021-10-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
160610_Greenberg_db9.fasta Fasta
160620_JJ1_AvrPTO_CA_InPlanta_PTMs.sptm Other
160620_JJ1_AvrPTO_Z3_InPlanta_PTMs.sf3 Other
160620_JJ1_AvrPTO_Z3_InPlanta_PTMs.sptm Other
160620_JJ2_LePTO_CA_InPlanta_PTMs.sptm Other
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