Proteomics

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Assessing the biomolecular landscape and dynamics of systemically circulating lipid-carrying particles


ABSTRACT: Separating lipid-carrying particles from body fluids is challenging and complicates studies of extracellular vesicles (EV) and lipoprotein particles (LPP) as disease biomarkers. In this study, we assess the biomolecular composition and dynamics of systemically circulating matched EV and LPP by analyzing serial blood plasma samples from breast cancer, ovarian cancer and HIV patients (n=36). Particles were separated through combined size-exclusion chromatography (SEC) and equilibrium density gradient centrifugation. After the analytical validity of our method is demonstrated, we reveal that EV carry a unique, dynamic and condition-dependent protein composition and RNA profile, which is not directly measurable in LPP extracts and total blood plasma, respectively. Our study extends the knowledge on the compositional nature of the EV protein corona and provides a catalog of proteins associated with systemically circulating EV and LPP. We anticipate that this research will steer the further development of EV-based biomarkers to detect and monitor disease.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Malignant Neoplasm Of Ovary,Human Immunodeficiency Virus Infectious Disease,Breast Cancer

SUBMITTER: Delphi Van Haver  

LAB HEAD: An Hendrix

PROVIDER: PXD023001 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions.

Vergauwen Glenn G   Tulkens Joeri J   Pinheiro Cláudio C   Avila Cobos Francisco F   Dedeyne Sándor S   De Scheerder Marie-Angélique MA   Vandekerckhove Linos L   Impens Francis F   Miinalainen Ilkka I   Braems Geert G   Gevaert Kris K   Mestdagh Pieter P   Vandesompele Jo J   Denys Hannelore H   De Wever Olivier O   Hendrix An A  

Journal of extracellular vesicles 20210814 10


Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size-exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical context-dependent and time-dependent variations in the biomolecular landscape of systemically circulating EV. Using pooled blood plasma samples from breast cancer patients, we first demonstrate the t  ...[more]

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