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Protein kinase C activation drives a differentiation program in an oligodendroglial precursor model through the modulation of specific biological networks

ABSTRACT: Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in a variety of cellular models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms that regulate oligodendrocytes differentiation after PKC activation are still unclear. Here we applied a liquid chromatography - mass spectrometry (LC-MS/MS) approach to identify proteins, and biological processes, modulated after PKC activation in the established PKC-induced differentiation model, MO3.13 oligodendroglial cell line. Our analysis revealed that PKC signaling activation leads to the down-regulation of biological processes related to cell cycle and DNA replication and the up-regulation of a glial like developmental program. Our data suggested a role for ROCK in the modulation of MO3.13 phenotype, cytoskeletal and mechanotransduction dynamics but not in the modulation of cell cycle and calcium transport. Overall, our findings provides a resource for elucidating the PKC-mediated signaling network contributing to a more robust knowledge of the molecular dynamics leading to this cell fate transition.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oligodendrocyte, Cell Culture

SUBMITTER: Marina Damato

LAB HEAD: Michel Salzet (Lab Head)

PROVIDER: PXD023336 | Pride | 2021-09-09

REPOSITORIES: Pride

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Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.

Damato Marina M Cardon Tristan T Wisztorski Maxence M Fournier Isabelle I Pieragostino Damiana D Cicalini Ilaria I Salzet Michel M Vergara Daniele D Maffia Michele M

International journal of molecular sciences 20210515 10

Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in various cell models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms regulating oligodendrocyte differentiation after PKC activation are still unclear. Here, we applied a liquid chromatography-mass spectrometry (LC-MS/MS) approach to provide a comprehensive analysis of the proteome expression changes in the MO3.13 oligodendroglial cell line after PKC activat ...[more]

PMID: 34063504

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			Action	DRS
	M03-13-Ctrl-1.raw	Raw
	M03-13-Ctrl-2.raw	Raw
	M03-13-Ctrl-3.raw	Raw
	M03-13-PMA_48H-1.raw	Raw
	M03-13-PMA_48H-2.raw	Raw