Dataset Information

Dictyostelium DIA LFQ proteomics of vegetative and developmental growth

ABSTRACT: Dictyostelium discoideum behavior depends on nutrients. When sufficient food is present these amoebae exist in a unicellular state (vegetative growth), but upon starvation they aggregate into a multicellular organism (developmental growth). For proteomics 30 minutes and eight hour cultures of D. discoideum under vegetative and starved growth conditions were compared by DIA-LFQ. This unique biology makes D. discoideum an ideal model for investigating how fundamental metabolism commands cell differentiation and function. We show here that reactive oxygen species (ROS), generated as a consequence of nutrient limitation, lead to the sequestration of the amino acid cysteine in the antioxidant glutathione, limiting the use of its sulfur atom for processes such as protein translation and FeS cluster-containing enzyme activity that contribute to mitochondrial metabolism and cellular proliferation. Such regulated sulfur sequestration maintains D. discoideum in a non-proliferating state that paves the way for multicellular development. This new mechanism of ROS signaling highlights oxygen and sulfur as simple, early evolutionary signaling molecules dictating cell fate, with implications for responses to nutrient fluctuations in higher eukaryotes.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Gerhard Mittler

LAB HEAD: Gerhard Mittler

PROVIDER: PXD023404 | Pride | 2021-03-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DIA_BKelly_Dictost_Dev30min_biorep1.htrms	Other
	DIA_BKelly_Dictost_Dev30min_biorep1.raw	Raw
	DIA_BKelly_Dictost_Dev30min_biorep2.htrms	Other
	DIA_BKelly_Dictost_Dev30min_biorep2.raw	Raw
	DIA_BKelly_Dictost_Dev30min_biorep3.htrms	Other

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Publications

Sulfur sequestration promotes multicellularity during nutrient limitation.

Kelly Beth B Carrizo Gustavo E GE Edwards-Hicks Joy J Sanin David E DE Stanczak Michal A MA Priesnitz Chantal C Flachsmann Lea J LJ Curtis Jonathan D JD Mittler Gerhard G Musa Yaarub Y Becker Thomas T Buescher Joerg M JM Pearce Erika L EL

Nature 20210224 7850

The behaviour of Dictyostelium discoideum depends on nutrients<sup>1</sup>. When sufficient food is present these amoebae exist in a unicellular state, but upon starvation they aggregate into a multicellular organism<sup>2,3</sup>. This biology makes D. discoideum an ideal model for investigating how fundamental metabolism commands cell differentiation and function. Here we show that reactive oxygen species-generated as a consequence of nutrient limitation-lead to the sequestration of cysteine i ...[more]

PMID: 33627869

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