Dataset Information

Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages

ABSTRACT: PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and potentially establishment of cell polarity during zygote development in the mosquito midgut, suggesting that chemical inhibitors of PP1 may be explored for blocking parasite transmission

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Berghei Anka

SUBMITTER: Andrew Bottrill

LAB HEAD: Dr. Rita Tewari

PROVIDER: PXD023571 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Details_for_proteomics_data_submission.docx	Other
	Experiment_MS18-174_3_P6tg-Gametocytes_from_OT_180904_MS18-174_P6tg-Gametocytes_3.mzid.gz	Mzid
	Experiment_MS18-174_3_P6tg-Gametocytes_from_OT_180904_MS18-174_P6tg-Gametocytes_3.mzid_Experiment_MS18-174_3_P6tg-Gametocytes_from_OT_180904_MS18-174_P6tg-Gametocytes_3.MGF	Mzid
	Experiment_MS18-174_9_WTGFP-Gametocytes_from_OT_180904_MS18-174_WTGFP-Schizonts_9.mzid.gz	Mzid
	Experiment_MS18-174_9_WTGFP-Gametocytes_from_OT_180904_MS18-174_WTGFP-Schizonts_9.mzid_Experiment_MS18-174_9_WTGFP-Gametocytes_from_OT_180904_MS18-174_WTGFP-Schizonts_9.MGF	Mzid

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Publications

Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages.

Zeeshan Mohammad M Pandey Rajan R Subudhi Amit Kumar AK Ferguson David J P DJP Kaur Gursimran G Rashpa Ravish R Nugmanova Raushan R Brady Declan D Bottrill Andrew R AR Vaughan Sue S Brochet Mathieu M Bollen Mathieu M Pain Arnab A Holder Anthony A AA Guttery David S DS Tewari Rita R

Communications biology 20210618 1

PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage durin ...[more]

PMID: 34145386

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