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Rituximab and obinutuzumab differentially hijack the B-cell receptor and NOTCH1 signaling pathways.

ABSTRACT: An LC-MS/MS based phosphoproteomics study that characterizes the signaling processes downstream of the B-cell receptor and how they are differentially regulated following treatment with anti-CD20 monoclonal antibodies in B-cell lymphoma.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, B-lymphocyte Cell Line, B-lymphocyte

DISEASE(S): B-cell Lymphoma,Diffuse Large B-cell Lymphoma

SUBMITTER: Arran Dokal

LAB HEAD: Pedro Cutillas

PROVIDER: PXD023572 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	F006652.mzid.gz	Mzid
	F006653.mzid.gz	Mzid
	F006654.mzid.gz	Mzid
	F006655.mzid.gz	Mzid
	F006656.mzid.gz	Mzid

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Publications

Rituximab and obinutuzumab differentially hijack the B cell receptor and NOTCH1 signaling pathways.

Edelmann Jennifer J Dokal Arran D AD Vilventhraraja Emma E Holzmann Karlheinz K Britton David D Klymenko Tetyana T Döhner Hartmut H Cragg Mark M Braun Andrejs A Cutillas Pedro P Gribben John G JG

iScience 20210122 2

The anti-CD20 monoclonal antibodies rituximab and obinutuzumab differ in their mechanisms of action, with obinutuzumab evoking greater direct B cell death. To characterize the signaling processes responsible for improved B cell killing by obinutuzumab, we undertook a phosphoproteomics approach and demonstrate that rituximab and obinutuzumab differentially activate pathways downstream of the B cell receptor. Although both antibodies induce strong ERK and MYC activation sufficient to promote cell- ...[more]

PMID: 33615197

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