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Genome-wide transcript and protein analysis reveals distinct features of aging in the mouse heart

ABSTRACT: Understanding the molecular mechanisms underlying age-related changes in the heart is challenging due to the contributions from numerous genetic and environmental factors. Genetically diverse outbred mice provide a model to study the genetic regulation of aging processes in healthy tissues from individuals undergoing natural aging in a controlled environment. We analyzed transcriptome and proteome data from outbred mice at 6, 12 and 18 months of age to reveal a scenario of cardiac hypertrophy, fibrosis, extracellular matrix remodeling, and reemergence of fetal gene expression patterns. We observed widespread changes in protein trafficking and sorting, and post-translational disruption of the stoichiometry of the protein quality control system itself. We identified genome hotspots of age-by-genetic effects that regulate proteins from the proteasome and endoplasmic reticulum stress response, suggesting that genetic variation in these modules may contribute to individual variation in the aging heart.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Joel Chick

LAB HEAD: Steven Gygi

PROVIDER: PXD023724 | Pride | 2021-12-23

REPOSITORIES: pride

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			Action	DRS
	233581_z06235_heart_aging_S01_F01.pep.xml	Pepxml
	233762_z06236_heart_aging_S01_F02.pep.xml	Pepxml
	233765_z06237_heart_aging_S01_F03.pep.xml	Pepxml
	233766_z06239_heart_aging_S01_F05.pep.xml	Pepxml
	233767_z06238_heart_aging_S01_F04.pep.xml	Pepxml

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