Proteomics

Dataset Information

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Label-free proteomic analysis of neuroglobin-knockout breast cancer cells


ABSTRACT: Neuroglobin (NGB) is an oxygen-binding protein that takes part in several processes that safeguard redox homeostasis, gaining an important protective role in neuronal and extra-neuronal cells. NGB remarkably exerts its function upon up-regulation by NGB-inducible molecules such as 17β-estradiol (E2) and H2O2. Despite this, the essential mechanisms related to NGB function remain still undefined. Human MCF-7 breast cancer cells knocked out (KO) for NGB gene obtained using CRISPR/Cas9 technology were analyzed by shotgun label-free quantitative proteomics in comparison with control cells. Differential proteomics experiments were also performed using NGB-KO cells after treatment with E2, H2O2, and E2+H2O2 versus their corresponding non-treated controls.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Michele Costanzo  

LAB HEAD: Michele Costanzo

PROVIDER: PXD023734 | Pride | 2021-07-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
190712_LFQ_n1-2_MaCA_KO-1.raw Raw
190712_LFQ_n1-2_MaCA_KO-2.raw Raw
190712_LFQ_n1-2_MaCA_KO-3.raw Raw
190712_LFQ_n1-2_MaCA_KO-4.raw Raw
190712_LFQ_n1-2_MaCA_KOE2-1.raw Raw
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Publications

Proteomic and Bioinformatic Investigation of Altered Pathways in Neuroglobin-Deficient Breast Cancer Cells.

Costanzo Michele M   Fiocchetti Marco M   Ascenzi Paolo P   Marino Maria M   Caterino Marianna M   Ruoppolo Margherita M  

Molecules (Basel, Switzerland) 20210420 8


Neuroglobin (NGB) is a myoglobin-like monomeric globin that is involved in several processes, displaying a pivotal redox-dependent protective role in neuronal and extra-neuronal cells. NGB remarkably exerts its function upon upregulation by NGB inducers, such as 17β-estradiol (E2) and H<sub>2</sub>O<sub>2</sub>. However, the molecular bases of NGB's functions remain undefined, mainly in non-neuronal cancer cells. Human MCF-7 breast cancer cells with a knocked-out (KO) <i>NGB</i> gene obtained us  ...[more]

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