Dataset Information

Borrelia burgdorferi infected Ixodes scapularis nymph saliva proteome

ABSTRACT: Lyme disease (LD) is one of the most important human vector-borne diseases in North America. Since transmission of Borrelia burgdorferi (Bb), the causative agent of LD, is influenced by yet unknown tick saliva proteins (TSP), the discovery and characterization of such TSPs are highly sought after for their potential as tick-antigen based vaccine targets. We validated a novel non-invasive approach of collecting sufficient amounts of saliva from unfed, partially fed (12, 24, 36, 48, 60, and 72h), and replete fed Ixodes scapularis nymphs for identification of tick saliva proteins using the LC-MS/MS approach. Our data has described TSPs that might be injected into the host within few hours of the tick starting to feed and are likely associated with manipulating host immunity to facilitate transmitted Bb to colonize the host. Thus, these data will serve as a source for Bb-infection associated TSPs that might be targeted in tick-antigen based vaccines to prevent LD. Equally important, the non-invasive method to collect nymph tick saliva is likely to provide transformative impact on research to understand transmission of other TBD agents.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Borreliella Burgdorferi Ixodes Scapularis (black-legged Tick) (deer Tick) Oryctolagus Cuniculus (rabbit)

TISSUE(S): Saliva

DISEASE(S): Lyme Disease

SUBMITTER: Albert Mulenga

LAB HEAD: Albert Mulenga

PROVIDER: PXD023940 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20170330_ISC-03_1-UF.ms2	Other
	20170330_ISC-03_1.mzXML	Mzxml
	20170330_ISC-03_1.mzid.gz	Mzid
	20170330_ISC-03_1.raw	Raw
	20170330_ISC-03_2-UF.ms2	Other

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Publications

Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs.

Kim Tae Kwon TK Tirloni Lucas L Bencosme-Cuevas Emily E Kim Tae Heung TH Diedrich Jolene K JK Yates John R JR Mulenga Albert A

BMC genomics 20210304 1

<h4>Background</h4>Lyme disease (LD) caused by Borrelia burgdorferi is the most prevalent tick-borne disease. There is evidence that vaccines based on tick proteins that promote tick transmission of B. burgdorferi could prevent LD. As Ixodes scapularis nymph tick bites are responsible for most LD cases, this study sought to identify nymph tick saliva proteins associated with B. burgdorferi transmission using LC-MS/MS. Tick saliva was collected using a non-invasive method of stimulating ticks (un ...[more]

PMID: 33663385

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