Proteomics

Dataset Information

0

Interaction of Hsp70 Ssb1 and atypical Hsp70 Ssz1


ABSTRACT: Our goal was to better understand the interaction between the Hsp70 Ssb1 and the atypical Hsp70 Ssz1, which we had uncovered by in vivo site-specific Bpa crosslinking. Ssz1 having Bpa incorporated was purified and incubated with purified Ssb1 and the J-domain protein Zuo1 during activation of photo cross-linking. After protein gel electrophoresis and protease digestion of excised bands, the resulting cross-linked peptides were analyzed by mass spectrometry and identified by StavroX.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture, Cell Line Cell

SUBMITTER: Greg Sabat  

LAB HEAD: Dr. Elizabeth A, Craig

PROVIDER: PXD024065 | Pride | 2021-08-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Bpa_CID_AspNtrypsin_011421.ssf Other
Bpa_CID_trypsin_011421.ssf Other
Craig_E_coli_S282Bpa_20210201.fasta Fasta
Craig_E_coli_Y351Bpa_20210201.fasta Fasta
Crosslinking-database_Craig_Ssz1_S282Bpa.fasta Fasta
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