Dataset Information

Secretome analysis of C-mannosyltransferase-deficient hiPSCs

ABSTRACT: C-mannosylation is a modification of tryptophan residues with a single mannose effecting protein folding, secretion and/or function. To date, only few proteins have been proven to be C-mannosylated and studies aiming at global assessment of protein C-mannosylation from cells or tissues are scarce. In order to interrogate the C-mannosylome of human induced pluripotent stem cells (hiPSCs), we made use of the common finding that C-mannosylation is important for protein secretion. Thus we compared the secretomes of C-mannosyltransferase-deficient DPY19L1 and DPY19L3 mutants to their parental wild-type hiPSCs by mass-spectrometry-based quantitative proteomics. Secretion of numerous proteins was reduced in the mutants.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Karsten Cirksena

LAB HEAD: Falk Büttner

PROVIDER: PXD024193 | Pride | 2021-06-17

REPOSITORIES: Pride

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Publications

The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development.

Cirksena Karsten K Hütte Hermann J HJ Shcherbakova Aleksandra A Thumberger Thomas T Sakson Roman R Weiss Stefan S Jensen Lars Riff LR Friedrich Alina A Todt Daniel D Kuss Andreas W AW Ruppert Thomas T Wittbrodt Joachim J Bakker Hans H Buettner Falk F R FFR

Molecular & cellular proteomics : MCP 20210508

C-mannosylation is a modification of tryptophan residues with a single mannose and can affect protein folding, secretion, and/or function. To date, only a few proteins have been demonstrated to be C-mannosylated, and studies that globally assess protein C-mannosylation are scarce. To interrogate the C-mannosylome of human induced pluripotent stem cells, we compared the secretomes of CRISPR-Cas9 mutants lacking either the C-mannosyltransferase DPY19L1 or DPY19L3 to WT human induced pluripotent st ...[more]

PMID: 33975020

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			Action	DRS
	200309_uniprot_homo_sapiens.fasta	Fasta
	FA05-18_Probe-1.raw	Raw
	FA05-18_Probe-10.raw	Raw
	FA05-18_Probe-11.raw	Raw
	FA05-18_Probe-12.raw	Raw