Proteomics

Dataset Information

0

SOX6 interacting proteins' identification in K562 cells for the regulation of γ-globin gene expression research

ABSTRACT: Human fetal γ-globin gene is developmentally silenced around the birth, and reactivation of γ-globin gene in adulthood sheds new light on ameliorating symptoms of hemoglobin disorders, such as sickle cell disease (SCD) and β-thalassemias. However, the precise regulation process of γ-globin remains incompletely understood. Here, we found that a new protein directly interacted with SOX6 and exerted a significant repression effect on the expression of γ-globin gene in erythroid cells. Further studies have demonstrated that it bound directly to γ-globin gene promoter via octamer binding motif, which in turn suppressed the transcriptional activity of γ-globin gene promoter. Thus, these data indicate that this new protein acts as a novel transcriptional repressor in the regulation of γ-globin gene expression through direct promoter binding, implying a potential alternative therapeutic target for the treatment of SCD and β-thalassemias.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

SUBMITTER: li xinyu  

LAB HEAD: Quan Zhao

PROVIDER: PXD024259 | Pride | 2022-02-16

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
K562.raw Raw
Proteins_results.xlsx Xlsx
mqpar.xml Xml
txt.zip Other
Items per page:
1 - 4 of 4
altmetric image

Publications

Human fetal globin (γ-globin) genes are developmentally silenced after birth, and reactivation of γ-globin expression in adulthood ameliorates symptoms of hemoglobin disorders, such as sickle cell disease (SCD) and β-thalassemia. However, the mechanisms by which γ-globin expression is precisely regulated are still incompletely understood. Here, we found that NonO (non-POU domain-containing octamer-binding protein) interacted directly with SOX6, and repressed the expression of γ-globin gene in hu  ...[more]

Similar Datasets

Transcriptomics Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease
2017-11-01 | GSE105035 | GEO
Proteomics Mass spec analysis of primary human erythroid cultures (healthy or thalassemic and treated or untreated with Decitabine)
2020-01-23 | PXD006889 | Pride
Genomics Natural regulatory mutations elevate fetal globin via disruption of BCL11A or ZBTB7A binding
2018-01-29 | GSE103445 | GEO
Other Potent and uniform fetal hemoglobin induction via base editing [Cut & Run]
2023-04-28 | GSE228819 | GEO
Proteomics The pivotal and universal role of BGLT3 lncRNA in fetal globin gene expression
2023-03-11 | PXD033536 | Pride
Transcriptomics bT87Q-globin gene therapy reduces sickle hemoglobin production, allowing for ex vivo anti-sickling activity in human erythroid cells
2020-04-13 | GSE148529 | GEO
Transcriptomics Distinct Ldb1/NLI complexes orchestrate γ-globin repression and re-activation through ETO2 in human adult erythroid cells.
2012-01-05 | E-GEOD-30047 | biostudies-arrayexpress
Proteomics Mitochondrial hTERT rewires glucose metabolism to confer cancer chemoresistance by binding to MT-ND1
2022-10-04 | PXD036126 | Pride
Proteomics Mass spectrum for identifying the interacting proteins of telomerase reverse transcriptase
2024-01-26 | PXD048859 | Pride
Genomics Distinct Ldb1/NLI complexes orchestrate γ-globin repression and re-activation through ETO2 in human adult erythroid cells.
2012-01-05 | GSE30047 | GEO