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Mass spec analysis of primary human erythroid cultures (healthy or thalassemic and treated or untreated with Decitabine)

ABSTRACT: Reactivation of gamma-globin is considered a promising approach for the treatment of beta-thalassaemia and sickle cell disease. Therapeutic induction of gamma-globin expression is fraught with lack of suitable therapeutic targets. In order to identify new potential targets we analysed the changes in the proteome of human primary erythroid progenitor cells by treatment with decitabine, a known, yet not clinically safe, gamma-globin inducer. Significant differentially expressed proteins were identified which were involved in various biological pathways and functional categories.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

DISEASE(S): Thalassemia

SUBMITTER: Eleni Katsantoni

LAB HEAD: Eleni Katsantoni

PROVIDER: PXD006889 | Pride | 2020-01-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Adria_1_3.raw	Raw
	Adria_1_4.raw	Raw
	Adria_1_ALL_NO_COISOLATION.msf	Msf
	Adria_1_REST.raw	Raw
	Adria_2_3.raw	Raw

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Publications

Proteomic Studies for the Investigation of γ-Globin Induction by Decitabine in Human Primary Erythroid Progenitor Cultures.

Theodorou Andria A Phylactides Marios M Katsantoni Eleni E Vougas Kostas K Garbis Spyros D SD Fanis Pavlos P Sitarou Maria M Thein Swee Lay SL Kleanthous Marina M

Journal of clinical medicine 20200103 1

Reactivation of γ-globin is considered a promising approach for the treatment of β-thalassemia and sickle cell disease. Therapeutic induction of γ-globin expression, however, is fraught with lack of suitable therapeutic targets. The aim of this study was to investigate the effects that treatment with decitabine has on the proteome of human primary erythroid cells from healthy and thalassemic volunteers, as a means of identifying new potential pharmacological targets. Decitabine is a known γ-glob ...[more]

PMID: 31947809

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