Dataset Information

Proteomic and pathway profiling of four Breast Cancer cell lines.

ABSTRACT: Biomarker discovery in breast cancer (BC) is a clinical need for therapeutics and non-invasive diagnostics. In a single study, we provide a comparative differential proteomic profile of four invasion, metastasis, angiogenesis and drug resistance. Exosome proteome profile reflects their different BC cell origin suggesting potential indicators of BC subtype. Further, our pathway inference analysis reveals a differential role of exosomes in BC signalling pathways in recipient cells, according to their protein cargo and cell origin. Our results may significantly contribute to further studies for the design of BC biomarker predictor to stratify BC patients and the development of novel therapeutic strategies.BC cell lines and their derived- exosomes, representative of the most relevant BC subtypes in clinic. Differentially-expressed proteins, in both cells and exosomes, include indicators of

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Breast Cancer

SUBMITTER: Luz Valero

LAB HEAD: María Jesús Vicent, Francisco García García

PROVIDER: PXD024304 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	QuantitationCellLines.xlsx	Xlsx
	SpectralLibrary_Cel-DDA.txt	Txt
	ZA-CEL-inGel-Lib.1.wiff	Wiff
	ZA-CEL-inGel-Lib.1.wiff.scan	Wiff
	ZA-CEL-inGel-Lib.2.wiff	Wiff

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Publications

Comparative profiling of whole-cell and exosome samples reveals protein signatures that stratify breast cancer subtypes.

Andreu Zoraida Z Hidalgo Marta R MR Masiá Esther E Romera-Giner Sergio S Malmierca-Merlo Pablo P López-Guerrero José A JA García-García Francisco F Vicent María J MJ

Cellular and molecular life sciences : CMLS 20240822 1

Identifying novel breast cancer biomarkers will improve patient stratification, enhance therapeutic outcomes, and help develop non-invasive diagnostics. We compared the proteomic profiles of whole-cell and exosomal samples of representative breast cancer cell subtypes to evaluate the potential of extracellular vesicles as non-invasive disease biomarkers in liquid biopsies. Overall, differentially-expressed proteins in whole-cell and exosome samples (which included markers for invasion, metastasi ...[more]

PMID: 39172142

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