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Drosophila hemolymph infected with Spiroplasma poulsonii

ABSTRACT: We investigated the effect of Spiroplasma infection on Drosophila hemolymph protein content using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). To this end, we extracted total hemolymph from uninfected and infected 10 days old females. At this age, Spiroplasma is already present at high titers in the hemolymph but does not cause major deleterious phenotypes to the fly. Extraction was achieved by puncturing the thorax and drawing out with a microinjector. Four replicates were made

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Hemolymph

SUBMITTER: Samuel Rommelaere

LAB HEAD: Bruno Lemaitre EPFL Switzerland

PROVIDER: PXD024340 | Pride | 2021-05-04

REPOSITORIES: Pride

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			Action	DRS
	Oxidation__M_Sites.txt	Txt
	Phospho__STY_Sites.txt	Txt
	SR_170224_HF_09.raw	Raw
	SR_170224_HF_10.raw	Raw
	SR_170224_HF_11.raw	Raw

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Publications

Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii.

Masson Florent F Rommelaere Samuel S Marra Alice A Schüpfer Fanny F Lemaitre Bruno B

PloS one 20210429 4

Insects are frequently infected with heritable bacterial endosymbionts. Endosymbionts have a dramatic impact on their host physiology and evolution. Their tissue distribution is variable with some species being housed intracellularly, some extracellularly and some having a mixed lifestyle. The impact of extracellular endosymbionts on the biofluids they colonize (e.g. insect hemolymph) is however difficult to appreciate because biofluid composition can depend on the contribution of numerous tissu ...[more]

PMID: 33914801

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