Proteomic Consequences of the Deletion of Cytochrome P450 (CYP450) Reductase in Mice
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ABSTRACT: Microsomal cytochrome P450 (CYP450) reductase enzymes play a major role in drug and xenobiotic metabolism. Mice which are deficient in hepatic CYP450 reductase serve as excellent models in understanding CYP450 drug metabolism and alterations in the underlying biology. A reversed-phase nano-bore UPLC-MS-based proteomic analysis, using an untargeted data independent approach (DIA), has been utilized for liver tissue extracts to evaluate differences between the proteomes of wild type (C57Bl6) and hepatic P450 reductase mice (HRNTM). Statistically curated, differential expressed protein groups highlighted a variety of molecular and biological functions, including binding and catalytic related activities. Pathway enrichment analysis resulted in perturbation of lipid and energy related pathways, which included bile acid biosynthesis, fatty acid omega oxidation and tricarbonic acid (TCA) cycle as examples.
INSTRUMENT(S): Synapt MS
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Liver
SUBMITTER: Lee Gethings
LAB HEAD: Lee Gethings
PROVIDER: PXD024420 | Pride | 2022-05-26
REPOSITORIES: Pride
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