Proteomics

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Analysis of the substrate specificities and reaction kinetics of the yeast oligosaccharyltransferase isoforms


ABSTRACT: Oligosaccharyltransferase (OST) catalyzes the central step in N-linked protein glycosylation, the transfer of a pre-assembled oligosaccharide from its lipid carrier onto asparagine residues of secretory proteins. The prototypic hetero-octameric OST complex from the yeast Saccharomyces cerevisiae exists as two isoforms that contain either Ost3p or Ost6p. These two OST complexes have different protein substrate specificities in vivo. The two OST complexes were purified from genetically engineered strains expressing only one isoform. The kinetic properties and substrate specificities were characterized using a quantitative in vitro glycosylation assay with short peptides and different synthetic lipid-linked oligosaccharide (LLO) substrates. We showed that the peptide sequence close to the glycosylation sequon affected peptide affinity and turnover rate. The length of the lipid moiety affected LLO affinity, while the lipid double bond stereochemistry had a greater influence on LLO turnover rates. The two OST complexes had similar affinities for both the peptide and LLO substrates but showed significantly different turnover rates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Diploid Cell

SUBMITTER: Chia-wei Lin  

LAB HEAD: Prof. Markus Aebi

PROVIDER: PXD024590 | Pride | 2021-05-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20180112_09_JE_OST6_1_F292945.mzid.gz Mzid
20180112_09_JE_OST6_EndoH.raw Raw
20180112_10_JE_OST3_EndoH.raw Raw
20180112_OST3_1_F292940.mzid.gz Mzid
20180711_14_JE_OST3_1_EndoH.raw Raw
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