Dataset Information

Control and Ikbkap-knockout MEFs LC-MSMS

ABSTRACT: Mouse Ikbkap gene encodes IKAP/Elp1- one of the core subunits of Elongator- and has been implicated in translational regulation. However, a role for IKAP in genome maintenance remains unclear. In this study, we analyze proteins from control and Ikbkap depletion mouse embryonic fibroblasts (MEF) using MS. Using MS-based proteomics, we show that several proteins involved in cancer and DNA damage response were found to be differentially expressed upon Ikbkap depletion.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Fibroblast, Fibroblast

DISEASE(S): Riley-day Syndrome

SUBMITTER: FU-JUNG LIN

LAB HEAD: Fu-Jung Lin

PROVIDER: PXD024628 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20201125_3650_NTUFJLin_CR0.raw	Raw
	20201125_3652_NTUFJLin_KO0.raw	Raw
	20201125_3654_NTUFJLin_CR4.raw	Raw
	20201125_3656_NTUFJLin_KO4.raw	Raw
	20201125_NTUFJLin_LFQ.msf	Msf

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Publications

Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.

Chen Wei-Ting WT Tseng Huan-Yi HY Jiang Chung-Lin CL Lee Chih-Ying CY Chi Peter P Chen Liuh-Yow LY Lo Kai-Yin KY Wang I-Ching IC Lin Fu-Jung FJ

Journal of biomedical science 20211124 1

<h4>Background</h4>RAD51-dependent homologous recombination (HR) is one of the most important pathways for repairing DNA double-strand breaks (DSBs), and its regulation is crucial to maintain genome integrity. Elp1 gene encodes IKAP/ELP1, a core subunit of the Elongator complex, which has been implicated in translational regulation. However, how ELP1 contributes to genome maintenance is unclear.<h4>Methods</h4>To investigate the function of Elp1, Elp1-deficient mouse embryonic fibroblasts (MEFs) ...[more]

PMID: 34819065

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