Dataset Information

Conditions for maintenance of hepatocyte differentiation and function in 3D cultures

ABSTRACT: 3D spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. However, the 3D spheroids are maintained under different conditions, with possible cofounding results. Here we performed an in-depth analysis of how various culture conditions influence 3D spheroids. Our aim was to find optimal conditions for the maintenance of a normal PHH phenotype.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Hepatocyte, Cell Culture

SUBMITTER: Niklas Handin

LAB HEAD: Per Artursson

PROVIDER: PXD024632 | Pride | 2022-02-17

REPOSITORIES: Pride

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Conditions for maintenance of hepatocyte differentiation and function in 3D cultures.

Handin Niklas N Mickols Evgeniya E Ölander Magnus M Rudfeldt Jakob J Blom Kristin K Nyberg Frida F Senkowski Wojciech W Urdzik Jozef J Maturi Varun V Fryknäs Mårten M Artursson Per P

iScience 20211005 11

Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different conditions, with possible confounding results. We performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an <i>in vivo</i> like phenotype. The formation, protein expression, and function of PHH spheroids were followed for three weeks in a high-throughput 384-well f ...[more]

PMID: 34746700

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