MTOR-mediated phosphorylation of VAMP8 prevents a starvation-induced VAMP8-SCFD1 interaction that promotes SNARE activity and autolysosome fusion
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
RONG LIU
LAB HEAD: RONG LIU
PROVIDER: PXD024642 | Pride | 2022-02-17
REPOSITORIES: Pride
Dataset's files
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| MS171518_V4.msf | Msf | |||
| MS171518_V4_20171113093700.raw | Raw | |||
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Publications
mTOR-mediated phosphorylation of VAMP8 and SCFD1 regulates autophagosome maturation.
Huang Hong H Ouyang Qinqin Q Zhu Min M Yu Haijia H Mei Kunrong K Liu Rong R
Nature communications 20211116 1
The mammalian target of rapamycin (mTORC1) has been shown to regulate autophagy at different steps. However, how mTORC1 regulates the N-ethylmaleimide-sensitive protein receptor (SNARE) complex remains elusive. Here we show that mTORC1 inhibits formation of the SNARE complex (STX17-SNAP29-VAMP8) by phosphorylating VAMP8, thereby blocking autophagosome-lysosome fusion. A VAMP8 phosphorylation mimic mutant is unable to promote autophagosome-lysosome fusion in vitro. Furthermore, we identify SCFD1, ...[more]
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