Proteomics

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Investigation of protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator

ABSTRACT: The aim of this study was to understand how autotrophic (CO2-fixing) bacteria balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment.To this end, the proteome of the model chemolithoautotroph Ralstonia eutropha a.k.a. Cupriavidus necator was studied in different environmental conditions (four limiting substrates, and five different growth rates). Cupriavidus was cultivated in substrate-limited chemostats with fructose, formate, succinate and ammonium limitation to obtain steady state cell samples. The dilution rate/growth rate was increased step-wise from 0.05 to 0.25 1/h in 0.05 steps. Protein quantity was determined by LC-MS, and enzyme utilization was investigated by resource balance analysis modeling.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Cupriavidus Necator (strain Atcc 17699 / H16 / Dsm 428 / Stanier 337) (ralstonia Eutropha)

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Michael Jahn  

LAB HEAD: Paul Hudson

PROVIDER: PXD024819 | Pride | 2022-02-17

REPOSITORIES: pride

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Protein allocation and utilization in the versatile chemolithoautotroph <i>Cupriavidus necator</i>.

Jahn Michael M   Crang Nick N   Janasch Markus M   Hober Andreas A   Forsström Björn B   Kimler Kyle K   Mattausch Alexander A   Chen Qi Q   Asplund-Samuelsson Johannes J   Hudson Elton Paul EP  

eLife 20211101

Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly versatile 'knallgas' bacterium <i>Cupriavidus necator</i> reallocates protein resources when grown on different limiting substrates and with different growth rates. We determined protein quantity by ma  ...[more]

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