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Advanced fiber type-specific protein profiles derived from adult murine skeletal muscle

ABSTRACT: Skeletal muscle is a heterogeneous tissue consisting of blood vessels, connective tissue, and muscle fibers. The last are highly adaptive and can change their molecular composition depending on external and internal factors, such as exercise, age, and disease. Thus, examination of the skeletal muscles at the fiber type level is essential to detect potential alterations. Therefore, we established a protocol in which myosin heavy chain isoform immunolabeled muscle fibers were laser microdissected and separately investigated by mass spectrometry to develop advanced proteomic profiles of all murine skeletal muscle fiber types. Our in-depth mass spectrometric analysis revealed unique fiber type protein profiles, confirming fiber type-specific metabolic properties and revealing a more versatile function of type IIx fibers. Furthermore, we found that multiple myopathy-associated proteins were enriched in type I and IIa fibers. To further optimize the assignment of fiber types based on the protein profile, we developed a hypothesis-free machine-learning approach (available at: https://github.com/mpc-bioinformatics/FiPSPi), identified a discriminative peptide panel, and confirmed our panel using a public data set.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle Fiber, Skeletal Muscle Tissue

SUBMITTER: Britta Eggers

LAB HEAD: Katrin Marcus

PROVIDER: PXD025359 | Pride | 2021-06-07

REPOSITORIES: Pride

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			Action	DRS
	QExHF04255.mzML	Mzml
	QExHF04255.raw	Raw
	QExHF04257.mzML	Mzml
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	QExHF04259.mzML	Mzml

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