Dataset Information

Label-free shotgun proteomics of M. hyopneumoniae and M. feriruminatoris

ABSTRACT: Mycoplasmas are wall-less parasitic bacteria possessing extremely small genomes. Despite this, growth rates differ among mycoplasma species with doubling times ranging from only 0.5h to 16h. Here, we analyzed the whole proteomes of M. hyopneumoniae and M. feriruminatoris and performed a comparative study in slow and fast growing mycoplasmas.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycoplasma Feriruminatoris Mycoplasma Hyopneumoniae (strain 232)

SUBMITTER: Marc Weber

LAB HEAD: Luis Serrano

PROVIDER: PXD025422 | Pride | 2021-08-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	160908_S_TFLS_10_03_2ug.raw	Raw
	160908_S_TFLS_10_04_2ug.raw	Raw
	161116_S_TFLS_05_01_2ug.raw	Raw
	161116_S_TFLS_05_02_2ug.raw	Raw
	180320_S_AMLS_05_01_1ug.raw	Raw

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Publications

Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control.

Burgos Raul R Weber Marc M Gallo Carolina C Lluch-Senar Maria M Serrano Luis L

iScience 20210816 9

Trans-translation is a ubiquitous bacterial mechanism of ribosome rescue mediated by a transfer-messenger RNA (tmRNA) that adds a degradation tag to the truncated nascent polypeptide. Here, we characterize this quality control system in a genome-reduced bacterium, <i>Mycoplasma pneumoniae</i> (MPN), and perform a comparative analysis of protein quality control components in slow and fast-growing prokaryotes. We show <i>in vivo</i> that in MPN the sole quality control cytoplasmic protease (Lon) d ...[more]

PMID: 34485867

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