Dataset Information

Proteomic Analysis of the Shandrin Mammoth by EVA Technology and High-Resolution Mass Spectrometry: What is its Gut Telling Us?

ABSTRACT: The investigation about archaeological samples, which are of inestimable value, requires minimally destructive or nondestructive sampling techniques. One of the most promising and recent non-invasive techniques is known under the acronym EVA, an ethylene vinyl acetate film which avoids contamination and proteins damages. This paper reports the results of the first-ever analysis of proteins extracted from the EVA film applied to a tissue sample some 40000 years old. In particular, the gut sample of a woolly mammoth (Mammuthus primigenus), discovered in 1972 close to the Shandrin River (Yakutia, Russia), via a shotgun MS-based approach was investigated. Proteomic and peptidomic analysis allowed the exploration of the mammoth dietary plants and its gut bacteria. The results were validated through the level of deamidation and other post-translational modifications of the peptides of the samples, which were used to discrimitate the “original” endogenous peptides from contaminant ones. Overall, the results of the metaproteomic analysis here reported are in accordance with the previous paleobotanical studies and with the reconstructed habitat of the Shandrin mammoth.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mammuthus Sp.

TISSUE(S): Gut Cavity

SUBMITTER: Vincenzo Cunsolo

LAB HEAD: Salvatore FotiLaboratory of Organic Mass Spectrometry, Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125, Catania, Italy

PROVIDER: PXD025518 | Pride | 2021-11-03

REPOSITORIES: Pride

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Publications

Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us?

Cucina Annamaria A Cunsolo Vincenzo V Di Francesco Antonella A Saletti Rosaria R Zilberstein Gleb G Zilberstein Svetlana S Tikhonov Alexei A Bublichenko Andrey G AG Righetti Pier Giorgio PG Foti Salvatore S

Amino acids 20210828 10

During the last decade, paleoproteomics allowed us to open a direct window into the biological past, improving our understanding of the phylogenetic relationships of extant and extinct species, past human diseases, and reconstruction of the human diet. In particular, meta-proteomic studies, mainly carried out on ancient human dental calculus, provided insights into past oral microbial communities and ancient diets. On the contrary, very few investigations regard the analysis of ancient gut micro ...[more]

PMID: 34453585

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			Action	DRS
	Mammoth_gut_Proteome_Xchange_Data_search.zip	Other
	est_bia_eva_1.raw	Raw
	est_bia_eva_2.raw	Raw
	est_bia_eva_3.raw	Raw
	gut_1.raw	Raw