Dataset Information

Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome inhibiting protein synthesis and reducing virulence

ABSTRACT: The antimicrobial properties of silver have been exploited for many centuries and continue to gain interest in the fight against antimicrobial drug resistance. The broad-spectrum activity and low toxicity of silver has led to its incorporation into a wide range of novel antimicrobial agents, including N-heterocyclic carbene (NHC) complexes. These versatile organic compounds readily bind transition metals and allow various substitutions, ring sizes and general properties. The antimicrobial activity and in vivo efficacy of the NHC silver(I) acetate complex SBC3, derived from 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*), has previously been demonstrated, although the mode(s) of action of SBC3 remained unclear. Label-free quantitative (LFQ) proteomics was employed to analyse protein abundance changes in the yeast Candida parapsilosis in response to SBC3 treatment and gain insight into potential SBC3 targets. Proteomic analysis of C. parapsilosis following exposure to SBC3 revealed increased abundance in proteins associated with cell wall synthesis, detoxification and drug efflux that are indicative of a cell stress response. Significant decreases in proteins required for protein synthesis, amino acid biosynthesis and respiration offer potential insight into the growth-inhibitory mechanisms of SBC3. Exposure of C. parapsilosis to SBC3 lead to reduced adherence to epithelial cells and biofilm formation which may indicate the compound can reduce fungal virulence.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Parapsilosis Cdc317

TISSUE(S): Cell Culture

SUBMITTER: Magdalena Piatek

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD025693 | Pride | 2022-06-28

REPOSITORIES: Pride

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Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence.

Piatek Magdalena M O'Beirne Cillian C Beato Zoe Z Tacke Matthias M Kavanagh Kevin K

Metallomics : integrated biometal science 20220801 8

The antimicrobial properties of silver have been exploited for many centuries and continue to gain interest in the fight against antimicrobial drug resistance. The broad-spectrum activity and low toxicity of silver have led to its incorporation into a wide range of novel antimicrobial agents, including N-heterocyclic carbene (NHC) complexes. The antimicrobial activity and in vivo efficacy of the NHC silver(I) acetate complex SBC3, derived from 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*), ...[more]

PMID: 35751649

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