Proteomics

Dataset Information

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Diversity of cationic antimicrobial peptides in black cumin (Nigella sativa L.) seeds


ABSTRACT: Black cumin (Nigella sativa L.) is known to possess a wide variety of antimicrobial peptides belonging to different structural families. Three novel antimicrobial peptides have been isolated from black cumin seeds. Two of them were attributed as members of the non-specific lipid transfer proteins family and one - as a defensin. We have made an attempt of using proteomic approach for novel antimicrobial peptides search in N. sativa seeds as well. The use of well established approach that includes extraction and fractionation stages remains relevant even in case of novel peptides search because the lacking of N. sativa genome data. Novel peptides demonstrate a spectrum of antimicrobial activity against plant pathogenic organisms that may cause economically important crop diseases. These results obtained allow considering these molecules as candidates to be applied in "next-generation" biopesticides development for agriculture use.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Nigella Sativa

SUBMITTER: Elena Petrenko  

LAB HEAD: Eugene Rogozhin

PROVIDER: PXD040611 | Pride | 2023-07-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
NsT_UZ_russia.raw Raw
experimentalDesignTemplate.txt Txt
mqpar.xml Xml
txt.zip Other
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Publications

Diversity of Cationic Antimicrobial Peptides in Black Cumin (<i>Nigella sativa</i> L.) Seeds.

Barashkova Anna S AS   Smirnov Alexey N AN   Zorina Elena S ES   Rogozhin Eugene A EA  

International journal of molecular sciences 20230429 9


Black cumin (<i>Nigella sativa</i> L.) is known to possess a wide variety of antimicrobial peptides belonging to different structural families. Three novel antimicrobial peptides have been isolated from black cumin seeds. Two of them were attributed as members of the non-specific lipid transfer proteins family, and one as a defensin. We have made an attempt of using the proteomic approach for novel antimicrobial peptides search in <i>N. sativa</i> seeds as well. The use of a well-established app  ...[more]

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