Dataset Information

Proteomic response to drought and Phytophthora cinnamomi in Quercus ilex.

ABSTRACT: Biotic and abiotic stresses are predicted to be the main drivers of forest tree decline and mortality in a climatic change scenario. It occurs, for example, in Quercus ilex, the main component of the Mediterranean forest, becoming a major environmental, economic and social concern. The decline syndrome is the result of factors interrelated in time and space. Breeding, through variability and elite genotype selection, is the only plausible strategy for forest management and conservation in nondomesticated, orphan species as is the case of the genus Quercus. By using a shotgun proteomics approach, the effect and the responses to combined drought stress and pathogen (Phytophthora cinnamomi) have been analyzed in two Holm oak populations with contrasting responses to each individual stress. Proteomics data are correlated with that of plant survival, damage symptoms, leaf chemical composition and physiological phenotypes (water content and photosynthetic activity). Changes in the protein profile depended on the population, stress, and time, with some consistent upaccumulated proteins related to synthesis, primary and secondary metabolism, and cell wall are discussed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Quercus Ilex (holly Oak)

TISSUE(S): Leaf

SUBMITTER: Bonoso San Eufrasio Martínez

LAB HEAD: Jesús V. Jorrín Novo

PROVIDER: PXD025704 | Pride | 2021-08-18

REPOSITORIES: Pride

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			Action	DRS
	MACastillejo_sol339-23-20_AC1_11112020.mgf	Mgf
	MACastillejo_sol339-23-20_AC1_11112020.raw	Raw
	MACastillejo_sol339-23-20_AC2_11112020.mgf	Mgf
	MACastillejo_sol339-23-20_AC2_11112020.raw	Raw
	MACastillejo_sol339-23-20_AC3_11112020.mgf	Mgf

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Project description:Proteases and protease inhibitors have been analyzed in the non-orthodox Quercus ilex forest tree by using in silico and wet approaches. The in silico analysis showed that the species-specific transcriptome database included 2240 and 97 transcripts annotated as, respectively, proteases and protease inhibitors, with members of the different families according to the MEROPS protease database (http://www.merops.ac.uk) classification, mostly of serine- and metallo-type. Data for Q. ilex are compared with those previously published for other Quercus species, namely, Q. suber, Q. lobata, and Q. robur. A second approach was performed to assess the evolution of proteases and protease inhibitors along with seed germination in cotyledon and embryo tissues by determining changes at proteomics and activity levels, the last by mean of in vitro and in gel assays. Shotgun (LC-MSMS) analysis of acorn embryos and cotyledons of three developmental stages (NV, non-viable; T1, mature; and T3 germinated) allowed the identification of a total of 177 proteases and 12 protease inhibitors. In vitro protease activity was determined by using azocasein as substrate; the total activity was higher in cotyledons than embryos, showing the latter significant differences throughout germination. Gel protease activity assays showed common and differential bands between tissues and developmental stages. Shotgun analysis of 10 differential activity bands supported the results obtained from the whole proteome analysis, with some proteins highly represented in the same tissues and stages by both strategies. In overall, the combination of shotgun proteomics and protease activity approaches allowed the identification of tissue-specific (such as cysteine protease inhibitors), and stage-specific proteins (such as those related to storage proteins mobilization), with others being present in all the analyzed organs. Some of the proteases and protease inhibitors differentially identified between tissues and stages in this study could be used as indicators of seed quality and viability.

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Proteomic response to drought and Phytophthora cinnamomi in Quercus ilex.

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