Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of the N- and C-terminal domain interface of the three main apoE isoforms

ABSTRACT: Chemical cross-linking coupled to mass spectrometry was used to investigate the conformations of the apolipoprotein isoforms apoE2, apoE3, and apoE4. Cross-linking data was generated using the reagents disuccinimidyl suberate (DSS), pimelic dihydrazide (PDH), and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride. Intramolecular interactions derived from the cross-linking experiments were combined with molecular modeling approaches.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Alexander Leitner

LAB HEAD: Alexander Leitner

PROVIDER: PXD025710 | Pride | 2025-01-26

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ApoE_file_list_PRIDE.xlsx	Xlsx
	Results_in_gel_dig.xlsx	Xlsx
	Results_in_solution_dig.xlsx	Xlsx
	checksum.txt	Txt
	mazadeh_C1509_001.raw	Raw

Items per page:

1 - 5 of 102

Similar Datasets

Structural analysis of the native TREX complex from S. cerevisiae

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the endogenously purified S. cerevisiae TREX complex. Cross-linking was performed using (a) bis(sulfosuccinimidyl) suberate (BS3) or (b) a combination of adipic dihydrazide (ADH) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride.

2023-09-15 | PXD025843 | Pride

Expanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 1: Model proteins

Project description:The conditions for chemical protein-protein cross-linking using the reagents pimelic dihydrazide (PDH) and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride were optimized using a set of five model proteins and two large complexes, the 20S proteasome and the 70S ribosome. This dataset contains data from the model proteins only.

2021-01-04 | PXD019724 | Pride

Structural architecture and function of the human NALCN channelosome

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the subunit organization of the NALCN channelosome consisting of the proteins NALCN, FAM155A, UNC79 and UNC80. Cross-linking data was generated using the reagents disuccinimidyl suberate (DSS), pimelic dihydrazide (PDH), and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride, and was analyzed in combination with data from cryo-electron microscopy.

2021-11-23 | PXD027213 | Pride

Expanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 3: E. coli 70S ribosome

2021-01-04 | PXD019508 | Pride

Expanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 2: Bovine 20S proteasome

2021-01-04 | PXD019507 | Pride

Molecular architecture of the human tRNA ligase complex

Project description:Chemical cross-linking coupled to mass spectrometry was used to study two tRNA ligase complexes, one consisting of subunits RTCB, DDX1, CGI-99, FAM98B and Ashwin (“full tRLC”) and one lacking Ashwin (“core tRLC”). Cross-linking was performed using the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2021-12-15 | PXD025662 | Pride

Puf6 ushers correct rRNA compaction during ribosome assembly

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the complex between the nucleolar factor, Puf6, and the yeast 60S ribosome. Disuccinimidyl suberate (DSS) was used as the cross-linking reagent.

2021-06-28 | PXD024131 | Pride

The TRiC Chaperonin and Prefoldin Cooperate to Fold and Assemble Viral Capsid Oligomers

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the folding of the reovirus sigma3 protein by the chaperonin, TRiC/CCT. Cross-linking was performed using the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2021-02-04 | PXD019751 | Pride

Characterization of Integrator module INTS10-INTS13-INTS14 and its connection to the cleavage module Part I

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the complex between the Integrator complex subunits INTS13 (Asunder) and INTS14. Cross-linking was performed using disuccinimidyl suberate (DSS).

2020-06-18 | PXD015682 | Pride

A structural mechanism for PP2A-B56alpha inhibition by CIP2A

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the structure of the oncoprotein CIP2A and its interaction with the B56alpha regulatory subunit of protein phosphatase 2A. Cross-linking was performed using disuccinimidyl suberate (DSS) or a combination of pimelic dihydrazide (PDH) and the coupling reagent DMTMM.

2022-10-21 | PXD020636 | Pride