Dataset Information

Effect of soybean agglutinin on biological function of gut

ABSTRACT: This study aims to investigate the effects of SBA on the cell cycle, apoptosis, and to verify the mechanism of SBA anti-nutritional characters based on proteomic-based analysis. The IPEC-J2 cell line was cultured with medium containing 0.0, 0.5 or 2.0 mg/mL SBA. With increasing SBA levels, the percentage of the cells at G0/G1 phase, cell apoptosis rates, expressions of Bax and p21, and the activities of Casp-3 and Casp-9 were increased, while cyclin D1 and Bcl-2 expressions were declined (p<0.05). The proteomic analysis showed that the numbers of differentially expressed proteins, induced by SBA, were mainly enriched in different pathways including DNA replication, base excision repair, nucleus excision repair, mismatch repair, amide and peptide biosynthesis, ubiquitin-mediated proteolysis, as well as structures and functions of mitochondria and ribosome. In conclusion, the anti-nutritional mechanism of SBA is a complex cellular process. Such process including DNA related activities; protein synthesis and metabolism; signal-conducting relation; as well as subcellular structure and function. This study provides comprehensive information to understand the toxic mechanism of SBA in monogastrics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Enterococcus Faecalis (streptococcus Faecalis) Acyrthosiphon Pisum (pea Aphid) Heliocidaris Erythrogramma (sea Urchin) Yarrowia Lipolytica (candida Lipolytica) Pan Troglodytes (chimpanzee) Pinus Radiata (monterey Pine) Helicobacter Pylori J99 (campylobacter Pylori J99) Halobacterium Salinarum Merluccius Bilinearis (silver Hake) Drosophila Melanogaster (fruit Fly) Hepatitis C Virus Subtype 1a Pongo Pygmaeus (bornean Orangutan) Ictalurus Punctatus (channel Catfish) Hordeum Vulgare (barley) Trypanosoma Brucei Arabidopsis Thaliana (mouse-ear Cress) Gadus Morhua (atlantic Cod) Rattus Norvegicus (rat) Caenorhabditis Elegans Solanum Tuberosum (potato) Arachis Hypogaea (peanut) Vigna Mungo (rice Bean) (black Gram) Nicotiana Tabacum (common Tobacco) Homo Sapiens (human) Bos Taurus (bovine) Gallus Gallus (chicken) Cicer Arietinum (chickpea) (garbanzo) Mus Musculus (mouse) Bombyx Mori (silk Moth) Candida Albicans (yeast) Brassica Oleracea Var. Botrytis (cauliflower) Taeniopygia Guttata (zebra Finch) (poephila Guttata) Saccharomyces Cerevisiae (baker's Yeast) Xenopus Laevis (african Clawed Frog) Sus Scrofa Domesticus (domestic Pig) Escherichia Coli Struthio Camelus (ostrich) Brassica Napus (rape) Staphylococcus Aureus Ricinus Communis Zea Mays (maize) Dictyostelium Discoideum (slime Mold) Olea Europaea (common Olive) Equus Caballus (horse) Pisum Sativum (garden Pea) Danio Rerio (zebrafish) (brachydanio Rerio) Oryza Sativa (rice) Rattus Rattus (black Rat) Triticum Aestivum (wheat) Macaca Mulatta (rhesus Macaque) Mesocricetus Auratus (golden Hamster) Cercopithecus Aethiops (green Monkey) (grivet) Vitis Vinifera (grape) Apis Mellifera (honeybee)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Mohammed Hamdy Farouk

LAB HEAD: Guixin Qin

PROVIDER: PXD025751 | Pride | 2021-05-04

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 11

Publications

Biological Mechanisms Induced by Soybean Agglutinin Using an Intestinal Cell Model of Monogastric Animals.

Pan Li L Liu Yan Y Lan Hainan H Bao Nan N Zhao Yuan Y Sun Hui H Qin Guixin G Farouk Mohammed Hamdy MH

Frontiers in veterinary science 20210602

Soybean agglutinin (SBA) has a toxic effect on most animals. The anti-nutritional mechanisms of SBA are not fully understood, in terms of cell survival activity and metabolism of intestinal cells. This study aims to investigate the effects of SBA on the cell cycle, apoptosis, and to verify the mechanism of SBA anti-nutritional characters based on proteomic-based analysis. The IPEC-J2 cell line was cultured with medium containing 0.0, 0.5, or 2.0 mg/mL SBA. With increasing SBA levels, the percent ...[more]

PMID: 34150879

Similar Datasets

Project description:The novel Traditional Chinese Medicine Ramulus Mori (Sangzhi) alkaloid tablets (SZ-A) are approved by The China National Medical Products Administration for the treatment of type 2 diabetes mellitus (T2DM). However, the extensive pharmacological characteristics and the underlying mechanism are unknown. This study investigated the mechanisms by which SZ-A ameliorates glucose metabolism in KKAy mice, an animal model of T2DM. Diabetic KKAy mice were treated intragastrically with SZ-A once daily for 8 weeks, after which glucose levels, lipid metabolism, gut microbiome, systemic inflammatory factors, luminal concentrations of short-chain fatty acids (fecal samples), and ileal proteomic changes were evaluated. The ileum tissues were collected, and the effects of SZ-A on pathological inflammatory damage were evaluated by hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry. The mRNA and protein expression levels of various inflammatory markers, including monocyte chemoattractant protein-1 and phosphorylated nuclear factor kappa B p65, were detected in the ileum tissues. SZ-A improved glucose metabolism with enhanced insulin response and elevated glucagon-like peptide 1 (GLP-1) during the glucose tolerance test in diabetic KKAy mice. Gut microbiota analysis demonstrated that SZ-A administration elevated the abundance of Bacteroidaceae and Verrucomicrobia, reduced the levels of Rikenellaceae and Desulfovibrionaceae; and increased the concentrations of fecal acetic and propionic acids compared to the diabetic model group. Additionally, SZ-A markedly improved ileal inflammatory injury and pro-inflammatory macrophage infiltration and improved intestinal mucosal barrier function in diabetic KKAy mice. SZ-A also attenuated the levels of circulating endotoxin, pro-inflammatory cytokines, and chemokines in the mice sera. Collectively, SZ-A ameliorated the overall metabolic profile including glucose and lipid metabolism in KKAy mice, which may be associated with an improvement in GLP-1 and insulin secretion, at least in part by modulating the gut microbiome and relieving the degree of ileal and systemic inflammation.

			Action	DRS
	F8044TQ_1.raw	Raw
	F8044TQ_2.raw	Raw
	F8044TQ_3.raw	Raw
	F8044TQ_4.raw	Raw
	F8044TQ_5.raw	Raw