Dataset Information

Salt stress downregulates 2-hydroxybutyrylation in Arabidopsis siliques

ABSTRACT: In this study, we compared the siliques from Arabidopsis thaliana under salt stress (Ss) with those in the control (Cs). The results showed that Khib was abundant in siliques. However, there were certain significant differences between the Ss and the Cs: Totally 3,810 normalized Khib sites on 1,254 proteins were identified in siliques under salt stress, and Khib was up-regulated at 96 sites on 78 proteins while down-regulated at 282 sites on 205 proteins in Ss silique. Among them, 13 proteins, including F4IVN6, Q9M1P5, and Q9LF33, had sites with the most significant regulation Khib modification. Bioinformatics analysis suggests that Khib mainly participates in glycolysis/gluconeogenesis and endocytosis. In particular, there were 117 Khib-modified proteins that were mapped to the protein interaction database, and the proteins with the most significant up-regulation of Khib sites were on P46422, O82514, Q38970, Q9LD57, and Q9STW6, while the most down-regulated sites were on Q9M9K1, Q9SF16, O24456, P83484, Q9FWA3, and P54609. In the KEGG pathway enrichment analysis, Khib-modified proteins were enriched in several pathways related to energy metabolism, including gluconeogenesis pathway, pentose phosphate pathway, and pyruvate metabolism.

INSTRUMENT(S): TMT quantitation analysis

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Silique

SUBMITTER: Geriqiqige Hong

LAB HEAD: Guojing Li

PROVIDER: PXD028116 | Pride | 2021-12-30

REPOSITORIES: Pride

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Salt stress downregulates 2-hydroxybutyrylation in Arabidopsis siliques.

Hong Geriqiqige G Su Xiaoyi X Xu Ke K Liu Bin B Wang Guangxia G Li Jing J Wang Ruigang R Zhu Mulan M Li Guojing G

Journal of proteomics 20210922

Lysine 2-hydroxyisobutyrylation (Khib) is one of the newly discovered post-translational modifications (PTMs) through protein acylation. It has been reported to be widely distributed in both eukaryotes and prokaryotes, and plays an important role in chromatin conformation change, gene transcription, protein subcellular localization, protein-protein interaction, signal transduction, and cellular proliferation. In this study, the Khib modification proteome of siliques from A. thaliana under salt s ...[more]

PMID: 34562664

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