Proteomics

Dataset Information

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Human cytomegalovirus-induced host protein citrullination is crucial for viral replication

ABSTRACT: Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activity promotes viral fitness. Citrullinome analysis reveals significant changes in deimination levels of both cellular and viral proteins, with interferon (IFN)-inducible protein IFIT1 being the most heavily deiminated one. As genetic depletion of IFIT1 strongly enhances HCMV growth, and in vitro IFIT1 citrullination impairs its ability to bind to 5’-pppRNA, we propose that viral-induced IFIT1 citrullination is a novel mechanism of HCMV evasion from host antiviral resistance. Overall, our findings point to a crucial role of citrullination in subverting cellular responses to viral infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Virginie Girault  

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD025803 | Pride | 2021-07-20

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20180403_QEp5_ViGi_SA_IFIT1-LysC.raw Raw
20180403_QEp5_ViGi_SA_IFIT1cit-LysC.raw Raw
CitrullinationSites.txt Txt
UP000005640_9606.fasta Fasta
allPeptides.txt Txt
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Publications

Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activit  ...[more]

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