Proteomics

Dataset Information

0

Human cytomegalovirus-induced host protein citrullination is crucial for viral replication

ABSTRACT: Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activity promotes viral fitness. Citrullinome analysis reveals significant changes in deimination levels of both cellular and viral proteins, with interferon (IFN)-inducible protein IFIT1 being the most heavily deiminated one. As genetic depletion of IFIT1 strongly enhances HCMV growth, and in vitro IFIT1 citrullination impairs its ability to bind to 5’-pppRNA, we propose that viral-induced IFIT1 citrullination is a novel mechanism of HCMV evasion from host antiviral resistance. Overall, our findings point to a crucial role of citrullination in subverting cellular responses to viral infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Virginie Girault  

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD025803 | Pride | 2021-07-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20180403_QEp5_ViGi_SA_IFIT1-LysC.raw Raw
20180403_QEp5_ViGi_SA_IFIT1cit-LysC.raw Raw
CitrullinationSites.txt Txt
UP000005640_9606.fasta Fasta
allPeptides.txt Txt
Items per page:
1 - 5 of 13
altmetric image

Publications

Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activit  ...[more]

Similar Datasets

Proteomics Human cytomegalovirus-induced host protein citrullination is crucial for viral replication
2021-07-20 | PXD025818 | Pride
Proteomics A streamlined data analysis pipeline for the identification of sites of citrullination
2021-11-25 | PXD027358 | Pride
Transcriptomics H3Cit26 ChIP-chip from MCF-7 cells
2012-09-04 | E-GEOD-32599 | biostudies-arrayexpress
Genomics H3Cit26 ChIP-chip from MCF-7 cells
2012-09-04 | GSE32599 | GEO
Proteomics A quantitative and site-specific atlas of the PADI4-induced citrullinome reveals widespread existence of citrullination
2024-01-16 | PXD038702 | Pride
Proteomics The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis
2018-02-28 | PXD003444 | Pride
Proteomics Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning
2022-01-12 | PXD023131 | Pride
Transcriptomics Role of NuRD subunits CHD3 and CHD4 in human melanoma cells (RNA-seq)
2019-11-03 | GSE134849 | GEO
Genomics Role of NuRD subunits CHD3 and CHD4 in human melanoma cells (ChIP-seq)
2019-11-03 | GSE134848 | GEO
Genomics PAD2-mediated citrullination contributes to efficient oligodendrocyte differentiation and myelination
2019-04-23 | GSE115929 | GEO