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In vivo labelling of sediments from Kamchatka Peninsula springs with FP-alkyne

ABSTRACT: This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment samples were collected and treated using the following three conditions. DMSO- treated control FP-alkyne labelled Samples for each condition were prepared in triplicate, resulting a total number of 6 samples per spring. Labelling was performed using 4 µM of the probe FP-alkyne and incubation for 2 h in the hot spring.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Hot Springs Metagenome

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD025833 | Pride | 2024-06-03

REPOSITORIES: Pride

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			Action	DRS
	ACE_0493_SN01.raw	Raw
	ACE_0493_SN02.raw	Raw
	ACE_0493_SN03.raw	Raw
	ACE_0493_SN07.raw	Raw
	ACE_0493_SN08.raw	Raw

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