Dataset Information

Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials

ABSTRACT: HKU1 is a human betacoronavirus and infects host cells via highly glycosylated spike protein (S). At present, N-glycosylation of HKU1 S has been reported, however, little is known about its O-glycosylation, which hinders an in-depth understanding of its biological functions. Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica materials (HBS). The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins. The application of the developed method to HKU1 S1 subunit resulted in 61 novel O-glycosylation sites, among which 56% were predicted to be exposed on protein outer surface. Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were also analyzed. The obtained O-glycosylation dataset would provide valuable insights for the understanding of the structure of HKU1 S.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Human Coronavirus Hku1 (isolate N1)

TISSUE(S): T Cell, Cell Culture

DISEASE(S): Severe Acute Respiratory Syndrome

SUBMITTER: Dongmei Fu

LAB HEAD: Dongmei Fu

PROVIDER: PXD025967 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20201015_HKU_1_2_rare.mgf	Mgf
	20201015_HKU_1_2_rare.mzid.gz	Mzid
	20201015_HKU_1_2_rare_1_.mgf	Mgf
	20201015_HKU_1_2_rare_1_.mzid.gz	Mzid
	20201015_HKU_1_2_rare_2_.mgf	Mgf

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Publications

Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.

Cui Yun Y Dong Xuefang X Zhang Xiaofei X Chen Cheng C Fu Dongmei D Li Xiuling X Liang Xinmiao X

Frontiers in chemistry 20210813

HKU1 is a human beta coronavirus and infects host cells <i>via</i> highly glycosylated spike protein (S). The N-glycosylation of HKU1 S has been reported. However, little is known about its O-glycosylation, which hinders the in-depth understanding of its biological functions. Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials. The enrichment method for O-glycopeptides with HBS was developed and validated usi ...[more]

PMID: 34485242

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