Proteomics

Dataset Information

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Proteomics analysis of cellular nucleic acid binding proteins


ABSTRACT: Organic solvents and Ti4+-IMAC capture of formaldehyde (FA) cross-linked cells followed by mass spectrometry analysis to identify nucleic acid binding proteins in human cells.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Huiyu Wang  

LAB HEAD: Hua Xiao

PROVIDER: PXD026035 | Pride | 2023-10-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
0.1__FA-1.raw Raw
0.1__FA-2.raw Raw
0.1__FA-3.raw Raw
0.2__FA-1.raw Raw
0.2__FA-2.raw Raw
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Publications

Deciphering Nucleic Acid Binding Proteome of Mouse Immune Organs Reveals Hub Proteins for Aging.

Wang Huiyu H   Zhang Yan Y   Wang Zeyuan Z   Zhang Lu L   Guo Miao M   Cao Chengxi C   Xiao Hua H  

Molecular & cellular proteomics : MCP 20230628 8


Profiling the nucleic acid-binding proteins (NABPs) during aging process is critical to elucidate its roles in biological systems as well as transcriptional and translational regulation. Here, we developed a comprehensive strategy to survey the NABPs of mouse immune organs by using single cell preparation and selective capture technology-based proteomics. Our approach provided a global view of tissue NABPs from different organs under normal physiological conditions with extraction specificity of  ...[more]

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