Dataset Information

Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America

ABSTRACT: Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Clostridioides Difficile Str. A-027

SUBMITTER: Glauber Wagner

LAB HEAD: Gerly Brito

PROVIDER: PXD026218 | Pride | 2021-07-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E2018-03-20-02a-ICC45-GB-rpt1-120min-5ul-Run1.raw	Raw
	E2018-03-20-02b-ICC45-GB-rpt1-120min-5ul-Run2.raw	Raw
	E2018-03-20-02c-ICC45-GB-rpt1-120min-5ul-Run3.raw	Raw
	E2018-03-20-03a-ICC45-GB-rpt2-120min-5ul-Run1.raw	Raw
	E2018-03-20-03b-ICC45-GB-rpt2-120min-5ul-Run2.raw	Raw

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Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities.

de Melo Pacífico Dvison D Costa Cecília Leite CL Moura Hercules H Barr John R JR Maia Guilherme Augusto GA Filho Vilmar Benetti VB Moreira Renato Simões RS Wagner Glauber G Domingues Regina Maria Cavalcanti Pilotto RMCP Quesada-Gómez Carlos C de Oliveira Ferreira Eliane E de Castro Brito Gerly Anne GA

Scientific reports 20210624 1

Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liqui ...[more]

PMID: 34168208

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