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Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain

ABSTRACT: Dopamine (DA) neurons modulate neural circuits and behaviors via dopamine release from expansive, long range axonal projections. The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. Here, we demonstrate APEX2 proximity labeling within genetically targeted neurons in the mouse brain, enabling subcellular proteomics with cell type-specificity. By combining APEX2 biotinylation with mass spectrometry, we mapped the somatodendritic and axonal proteomes of DA neurons. Our dataset reveals the proteomic architecture underlying axonal transport, dopamine transmission, and axonal metabolism in DA neurons. We find a significant enrichment of proteins encoded by Parkinson’s disease-linked genes in dopaminergic axons, including proteins with previously undescribed axonal localization. Our proteomic datasets comprise a significant resource for axonal and DA neuronal cell biology, while the methodology developed here will enable future studies of other neural cell types.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Dopaminergic Neuron

DISEASE(S): Disease Free

SUBMITTER: Peter Sims

LAB HEAD: Peter Sims

PROVIDER: PXD026229 | Pride | 2021-06-01

REPOSITORIES: Pride

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			Action	DRS
	BH1046_Fusion_A1P2_Apex_P2_S28.raw	Raw
	BH1046_Fusion_A2P2_Apex_P2_S29.raw	Raw
	BH1046_Fusion_A3MFB_APEX_MFB_S13.raw	Raw
	BH1046_Fusion_A3P2_Apex_P2_S30.raw	Raw
	BH1046_Fusion_A3Str_APEX_Str_S21.raw	Raw

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