Unknown,Transcriptomics,Genomics,Proteomics

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The transcription factor nurr1 exerts concentration-dependent effects on target genes mediating distinct biological processes


ABSTRACT: The transcription factor nurr1 plays a pivotal role in the development and maintenance of neurotransmitter phenotype in midbrain dopamine neurons. Conversely, decreased nurr1 expression is associated with a number of dopamine-related CNS disorders, including Parkinson’s disease and drug addiction. In order to better understand the nature of nurr1-responsive genes and their potential roles in dopamine neuron differentiation and survival, we used a neural cellular background in which to generate a number of stable clonal lines with graded nurr1 gene expression that approximated that seen in DA cell-rich human substantia nigra. Gene expression profiling data from these nurr1-expressing clonal lines were validated by quantitative RT-PCR and subjected to bioinformatic analyses. The present study identified a large number of nurr1-responsive genes and demonstrated the potential importance of concentration-dependent nurr1 effects in the differential regulation of distinct nurr1 target genes and biological pathways. These data support the promise of nurr1-based CNS therapeutics for the neuroprotection and/or functional restoration of DA neurons. Total RNA obtained from nurr1-overexpressing SKNAS neuroblastoma clonal cell lines (SKNAS_E & SKNAS_G) compared to empty vector transfected control (SKNAS_C)

ORGANISM(S): Homo sapiens

SUBMITTER: Magen Johnson 

PROVIDER: E-GEOD-33434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Transcription Factor NURR1 Exerts Concentration-Dependent Effects on Target Genes Mediating Distinct Biological Processes.

Johnson Magen M MM   Michelhaugh Sharon K SK   Bouhamdan Mohamad M   Schmidt Carl J CJ   Bannon Michael J MJ  

Frontiers in neuroscience 20111220


The transcription factor NURR1 plays a pivotal role in the development and maintenance of neurotransmitter phenotype in midbrain dopamine neurons. Conversely, decreased NURR1 expression is associated with a number of dopamine-related CNS disorders, including Parkinson's disease and drug addiction. In order to better understand the nature of NURR1-responsive genes and their potential roles in dopamine neuron differentiation and survival, we used a human neural cellular background (SK-N-AS cells)  ...[more]

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