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SCFFbxw5 targets kinesin-13 proteins to facilitate ciliogenesis

ABSTRACT: Using substrate screening on protein microarrays we identified the kinesin-13 Kif2c/MCAK as a novel target of the ubiquitin E3 ligase SCF-Fbxw5. In cells, SCF-Fbxw5 ubiquitylates MCAK for proteasomal degradation during G2/M thereby facilitating ciliogenesis in the following G0 phase. Here, we performed mass spectrometry analysis to identify lysine residues of recombinant MCAK that are specifically ubiquitylated in vitro by SCF-Fbxw5 in concert with the E2 enzyme Cdc34 based on diGly remnants after trypsin digestion.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Thomas Ruppert

LAB HEAD: Thomas Ruppert

PROVIDER: PXD026353 | Pride | 2021-08-12

REPOSITORIES: pride

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			Action	DRS
	21-37_Melchior.pdf	Pdf
	Q25070-21-37-03.raw	Raw
	Q25070-21-37-04.raw	Raw
	Q25070-21-37-laneA_PSMs.xlsx	Xlsx
	Q25070-21-37-laneA_protein_groups.xlsx	Xlsx

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Publications

SCF<sup>Fbxw5</sup> targets kinesin-13 proteins to facilitate ciliogenesis.

Schweiggert Jörg J Habeck Gregor G Hess Sandra S Mikus Felix F Beloshistov Roman R Meese Klaus K Hata Shoji S Knobeloch Klaus-Peter KP Melchior Frauke F

The EMBO journal 20210809 18

Microtubule depolymerases of the kinesin-13 family play important roles in various cellular processes and are frequently overexpressed in different cancer types. Despite the importance of their correct abundance, remarkably little is known about how their levels are regulated in cells. Using comprehensive screening on protein microarrays, we identified 161 candidate substrates of the multi-subunit ubiquitin E3 ligase SCF<sup>Fbxw5</sup> , including the kinesin-13 member Kif2c/MCAK. In vitro reco ...[more]

PMID: 34368969

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Mass-spectrometry based quantification of HYPK, NAA10 and NAA15 protein steady-state levels in Arabidopsis thaliana mutants carrying a T-DNA insertion in the HYPK gene.

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